Biochem 5615 Weeks 1-2

Adenine

Guanine

Hypoxanthine

Inosine monophosphate

Xanthine

Cytosine

Uracil

What is another word for Uracil?

Orotate

thymine

Synthesis from amino acids, ribose-5-phosphate, CO2, and NH3

De novo

How to form AMP?

IMP + Asp + GTP→ GDP + P + intermediate→(remove fumarate)

How to form XMP?

IMP + H2O + NAD+ → NADH + H + ___

How to form GMP?

XMP + (Gln + H2O) + ATP → AMP + PP + ___

PRPP

Ribose 5- phosphate → ___

Ribose 5- phosphate → PRPP (inhibited by what?)

ADP, GDP

PRPP → phosphoribosyl amine (inhibited by what?)

IMP, AMP, GMP

PRPP → phosphoribosyl amine (activated by what?)

PRPP

phosphoribosyl amine → ___

IMP

IMP → adenylosuccinate (inhibited by what?)

AMP

IMP → xanthylate (inhibited by what?)

GMP

HCO3-+ 2ATP+ Gln → carbamoyl phosphate + Glu+ 2ADP+P

CPS II

Carbamoyl phosphate + Asp → carbamoyl aspartate

ATCase

ATCase inhibited by

CTP

ATCase activated by

ATP

Carbamoyl phosphate → H2O + ____

Dihydroorotate

Dihydroorotate + Q → QH2 + ___

Orotate

Orotate + ____ → PP + ___

PRPP, OMP

OMP → CO2 + UMP

OMP decarboxylase

OMP → CO2 + ___

UMP

UMP is inhibited by

CMP

Nucleoside monophosphate kinases

NMP kinase

AMP + ATP → 2 ADP

adenylate kinase

CPSII activated by

ATP, PRPP

CPSII inhibited by

UDP, UTP

Recycle free bases and nucleosides (mostly from diet)

Salvage

From our 5 carbon sugars we become

PRPP

what is pink?

glycine

what is green?

Amide N from glutamine

what is blue?

formate

what is yellow?

aspartate

what is purple?

CO2

what pathway is PRPP from?

Pentose phosphate pathway