PCOL 2 LAB: Husbandry and Management

• stable and uniform

• high reproducibility

inbred advantages

• genetically s___ and u___

• low/high reproducibility of results 

• poor model

• more expensive

• more sensitive

• low reproductive ability

inbred disadvantages

• poor/good model of human genetic diversity

• less/more expensive

• less/more sensitive

• less/high reproductive ability

• diverse

• good models

• drug safety testing

• less sensitive

• more robust

• high reproductive ability

outbred advantages

• genetically d___

• poor/good models for human genetic diversity 

• good for pharmacology and d___ s___ t___

• less/more sensitive

• less/more robust

• less/high reproductive ability

germ-free or axenic

ecological class: free of all microorganisms including those typically found in the gut

1. germ-free/axenic

2. gnotobiotic or defined flora

3. specific pathogen free (SPF)

4. conventional

4 ecological classes

1. GF/A

2. G/DF

3. SPF

4. C

germ-free or axenic and gnotobiotic or defined flora

ecological class: maintained in isolators & strict SOP

germ-free or axenic

ecological class: produced via IVF, embryo transfer, hysterectomy, rederivation

gnotobiotic or defined flora

ecological class: germ-free but intentionally seeded with 1 or more known non-pathogen or microorganisms

conventional

ecological class: colonized with a diverse and largely undefined microbiome (containing ~10¹¹ bacteria within the gut alone)

specific pathogen free (SPF)

ecological class: contain specifically defined list of pathogens through routine testing (specific microbiomes, opportunistic bacteria, commensals excluded from them)

~10¹¹

conventional class are colonized with a diverse and largely undefined microbiome (containing ___ bacteria within the gut alone)

malocclusion

dental disorder characterized by improper alignment of the teeth

pellets

required to prevent malocclusion

malocclusion

triggered/caused by non-pelleted or soft/liquid diets

chow diets

standard diets in most laboratory facilities

chow diets

diet produced from natural ingredients

chow diets

diet that is pelleted

chow diets

diet that have closed formulas /propriety

purified or semi-purified diets

diet that is combination of natural ingredients, pure nutrients and ingredients of varying degrees of refinement

purified or semi-purified diets

diet that may not be pelleted

purified or semi-purified diets

diets that may have open formulas

extruded diets

diets that are softer composition, less dense than pellets

extruded diets

diets that provide greater availability of nutrients

extruded diets

diets that may increase wastage

extruded diets

diets that have high production cost

• mice = 10-15 g

• rat = 10 g

normal average feed intake

• mice = ___-___ g/100g BW/day

• rat = ___g/100g BW/day

10%

drinking

• fed on dry diets - require ___% of the BW per 24 hours