concentration

Concentration of 1 molecule in E.coli volume

10**-9 (nM)

Concentration of ribosomes in E.coli

10**-5 - 10**-4

Total protein concentration

10**-1 =0,1 M

How far apart are molecules at 1 M concentration

1,18 nm

How far apart are molecules at 1 micromol. conc?

118nm

Formula for average separation

d = V**1/3 = 1.18/C**1/3

Concentration of hemoglobin in RBC

330 mg/mL = 5 mM

How many atoms there are in the human body?

10**27 - 10**28

The most common elements in human body?

H, O, C, N

Density of DNA compared to water?

2.0

Density of E.coli compared to water?

1.08 - 1.10

The most common metabolite?

Glutamate

The most common type of membrane lipids in mammalian cell?

Glycerophospholipid

How many proteins there are in the E. coli cell?

3× 10**6 proteins/μm

How many proteins there are in Hela cell line in a cell?

10×10**9

How many proteins there are in the human cell?

0.95-1.7×10**9

How many mRNA copies are there in bacterial cell?

10**3 - 10**4

How many mRNA copies there are in mammalian cell?

10**5 - 10**6

How many mRNA copies per gene on average in E.coli?

1

Protein/mRNA

1000-10000

The number of copies of activators per cell

1-100

Numbers of repressor copies per cell

10-1000

Percentage of dry cell mass of E.coli that proteins take

55%

How many ribosomes does the cell contain if it doubles every 30 min and every 40 min

45 000

26 000