Concentration technique

light infection

concentration techniques are techniques used in the examination of parasites in stool in cases of -x- that may have yielded a negative direct fecal smear reading, can be performed on fresh or preserved stool specimens.

protozoan cysts, coccidian cysts, miscrosporidia spores, and helminth eggs and larvae

designed to facilitate the recovery of?

trophozoites

-x- usually do not survive the procedure

Purpose

Aggregate parasites present into a small volume of the sample and to remove as much debris as possible that might hinder the laboratory technologist’s ability to see any parasites present clearly.

Flotation and Sedimentation Methods

what are the two types of concentration techniques?

1. Brine Flotation Method (By Willis)

2. Zinc Sulfate Flotation Technique

3. Sheather’s Sugar Flotation Technique

Enumerate the 3 flotation methods

1. Simple Gravity Sedimentation Technique

2. Acid Ether Concentration Technique (AECT)

3. Formalin Ether/ Ethyl Acetate Concentration Technique (FECT)

4. Merthiolate-Iodine Formalin Ether/Ethyl Acetate Concentration Technique (MIFECT)

5. Merthiolate Iodine Formalin Ether/ Formalin Concentration Technique (MIFCT)

6. Baermann Concentration Method

Enumerate the Sedimentation Methods

Specific gravity

ratio/ comaprison between reference substance and that of water

performing of the two methods

what is ideal but impractical?

Sedimentation methods

what is more recommended because they are more efficient and easier to perform accurately?