Extra Credit Quiz

Lab Syllabus: 3 Questions, Microscope Review: 4 Questions, Study Guide: 3 Questions

Study Guide: Practice using the microscope and survey some slides seen throughout this course:

• Letter ‘E’

• Kidney

• Skin

• Small Intestine Composite

Study Guide: Practice using the microscope to view the following slide under OIL IMMERSION

• Blood-smear

Study Guide: Explore the side bench of some of the materials used for histological technique

• Microtome

• Xylene: Removes alcohol from tissue, makes transparent

• Microscope slides and coverslip

• Ethanol: dehydrates tissue

• Hematoxylin and Eosin (H & E)

  • Hematoxylin: Blue: nucleus, acidic regions of the cytoplasm, cartilage matrix

  • Eosin: Pink: Pink: basic regions of the cytoplasm, collagen fibers

• Coplin Staining Jar

Study Guide: Explore the SECTRA table to learn how it works and to review the anatomical planes:

• Frontal (Coronal)

• Sagittal/Mid-Saggital

• Transverse (Cross-Section)

• Oblique

Study Guide: Explore the other SECTRA table to see how Histology Guide works

https://www.histologyguide.com

Lab Syllabus: Name, Email, Office Hours

Hector Aviles, [e-mail], Wednesday 1-2pm

Lab Syllabus: How many lab quizzes and practicals?

• 8 lab quizzes

  • 30 pts each

  • lowest lab score dropped

• 3 practicals

  • 75 pts each

Lab syllabus: What is the attendance/tardiness policy?

• Attendance: make up labs may not be possible after the Friday of each week

• Make up must be completed either before conflict or as soon as possible afterwards

Lab syllabus: What is the lab safety policy?

• No food or drinks

• Lab dress code:

  • Cover feet

  • Shoulders covered

  • Thighs covered

Lab syllabus: What lab materials are offered?

• Microscope: Olympus CX31

• SECTRA Table

• VH Dissector

Microscope Review: Define the microscope concept of “resolution”

Ability of a microscope to distinguish details of a specimen/slide

Microscope Review: What is the magnification power of the ocular lens (eyepiece)? What are the magnification powers of all four objective lenses?

• Eyepiece: 10x

• Objective lenses: 4x, 10x, 40x, 100x

Microscope Review: What are the total magnifications when viewing a specimen under each of the four objective lenses?

40x, 100x, 400x, 1000x

Microscope Review: Which objective lenses should you start with when looking at a specimen? Should the stage be all the way up or down?

• Objective lense with the lowest magnification power

• Stage should be all the way up

Microscope Review: Which part of the microscope moves the stage horizontally? Which part moves the stage vertically?

• Horizontally: Stage control knob

• Vertically: Course & fine adjustment knob

Microscope Review: he compound light microscopes utilized in the lab throughout the semester have parfocal objectives. Is this a useful feature?

Yes. It keeps the specimen in focus when switching magnification lenses.

Microscope Review: Walk the student through how to focus the compound light microscope in order to get to the point where they are clearly viewing the specimen with a high-power objective lense.

1. Turn the nosepiece so the lower power objective lens is in viewing position. Turn the adjustment knob so it is in the highest position (without touching the slide)

2. Look through eyepiece and turn adjustment knob slowly so stage moves down. Turn stage control as needed.

3. Once a clear image with the low-power objective is achieved, turn the nose piece to the next higher objective lens and adjust both the stage control and course adjustment knob as needed to get a clear image of the specimen.

Microscope Review: Walk student through how to view specimen under oil immersion and how to properly clean up.

1. Ensure slide is clean.

2. Place a drop of immersion oil on the area of the slide to be observed. Place the slide onto the stage and center it using the stage control knob.

3. Rotate the 100x oil immersion objective lens to the viewing position.

4. Raise the stage slowly so the 100x immersion objective is touching the drop of oil. Turn the fine focus knob to get the specimen into focus.

5. To clean, wipe the oil off from the slide and objective lens.