stank micro

Blood Agar-ESD

Enriched and differential

Blood agar enrichment

5% sheep blood

Blood agar differential

5% sheep blood, hemolysis of RBC

can have beta ( staph aureus)

alpha (strep pneumo)

none (klebsiella)

Chocolate Agar ESD

Enriched

Chocolate agar enrichment

NAD (V), Hemin (X), h. parainfluenzae- V, H. influenzae XV, H. ducreyi X

Chocolate agar sites

wounds, ears, eyes, sputum

Phenylethyl Alcohol Agar ESD

Enriched and selective

Phenylethyl Alcohol Agar enrichment

5% sheep blood

Phenylethyl Alcohol Agar Selection

phenylethyl alcohol inhibits gram negative organisms

Phenylethyl Alcohol Agar sites

wounds, anaerobic jars

MacConkey ESD

selective and differential

MacConkey Selection

crystal violet and bile salts inhibit gram positive organisms

MacConkey Differential

Lactose fermentation

fermenters: pink colonies

non fermenters: yellow colonies

MacConkey sites

wounds, sputum, ears, urines

Mannitol Salt Agar ESD

Selective and differential

Mannitol Salt Agar- Selection

High salt concentration, limits growth of most organisms except for halophiles

Mannitol Salt Agar- Diff

fermentation of mannitol

Fermenters: yellow colonies

non fermenters: pink colonies

Mannitol Salt Agar- Sites

nasal swabs to detect staph. aureus

Colistin Nalidixic acid Agar- ESD

Enriched and selective

Colistin Nalidixic acid agar- Enrichment

5% sheep blood

Colistin Nalidixic acid agar- selection

Colistin and Nalidixic acid inhibit gram negative bacteria

Colistin Nalidixic Acid agar- isolation of

Streptococcus and staphlococcus

Thayer Martin & Martin Lewis agar- ESD

Enriched and Selective

Thayer Martin & Martin Lewis agar- Enrichment

NAD+ and Hemin (same base as chocolate)

Thayer Martin & Martin Lewis agar- Selection

Colistin inhibits gram negative organisms

Vancomycin inhibits gram positive organisms

modified base to inhibit proteus

Thayer Martin & Martin Lewis agar- sites

cervical, rectal, penile, throat swabs

NYC Agar - ESD

Enriched and Selective

NYC agar- Enrichment

Peptone-cornstarch agar base, horse plasma

NYC agar- Selection

Vancomycin inhibits gram positive organisms

Colistin inhibits gram negative organisms

Trimethoprim inhibits proteus

Nyastin inhibits fungal elements

NYC agar isolation

neisseria gonorrhoeae

NYC agar sites

cervical, rectal, penile, throat swabs

Hektoen Enteric Agar -ESD

Selective and differential

Hektoen Enteric Agar- Selection

bile salts inhibit gram positive organisms

Hektoen Enteric Agar- Diff

Bunch of sugar fermentation

fermenters : pink, orange, yellow colonies

non-fermenters, H2S producing: clear colonies with black precipitate

non fermenters non H2S producing: clear colonies

Hektoen Enteric Agar isolation

H2S producing: Salmonella

Non H2S producing: Shigella

Hektoen Enteric Agar- Sites

Stool samples only

Salmonella Shigella agar- ESD

selective and differential

Salmonella Shigella Selection

bile salts, sodium citrate inhibit gram positive organisms

Sorbitol MacConkey agar- ESD

Selective and differential

Sorbitol MacConkey agar- Selection

Crystal violet, bile salts inhibit gram positive organisms

Sorbitol MacConkey agar- diff

Sorbitol fermentation

fermenters: pink colonies

non fermenters: yellow colonies

Sorbitol MacConkey agar- isolation

Fermenters: enterobacterales, other ecoli

non-fermenters: Ecoli O157 H7

Sorbitol MacConkey agar- sites

stool samples only

Cefsulodin-irgasan-novobiocin agar- ESD

selective and differential

Cefsulodin-Irgasan-Novobiocin agar Selection

Cefsulodin, Irgasan, Novobiocin, crystal violet and bile salts inhibit gram positive

Cefsulodin-Irgasan-Novobiocin agar diff

Mannitol fermentation

fermenter: clear colonies, red bullseye

non fermenter: clear colonies

Cefsulodin-Irgasan-Novobiocin agar isolation

Yersinia and Aeromonas

Cefsulodin-Irgasan-Novobiocin agar sites

stool samples only

Karmali Agar ESD

Enriched and selective

Karmali Agar enrichment

hemin

Karmali agar selection

vancomycin inhibits gram positive

cefoperazone inhibits gram negative

cycloheximide inhibits fungal elements

Karmali agar isolation

campylobacter

Karmali agar sites

stool samples only

GN broth & Selenite Broth ESD

Enriched and selective

GN broth & Selenite Broth Enrichment

enzymatic casein digest

GN broth & Selenite Broth Selection

Sodium selenite inhibits both gram positive and gram negative organisms

GN broth & Selenite Broth Isolation

Salmonella and shigella Plate on HEK after

GN broth & Selenite Broth sites

stool samples only, plated on HEK after

LIM broth ESD

Enriched and selective

LIM broth enrichment

peptones, dextrose, yeast extracts

LIM broth Selection

Colistin, nalidixic acid inhibits gram negative organisms

LIM broth Isolation

group B strep ( strep agalactiae)

LIM broth sites

cervical, rectal swabs

pregnant women

plated after

Thioglycolate broth -ESD

enriched and differential

Thioglycolate broth- enrichment

enzymatic digest of casein, dextrose, yeast extract

Thioglycolate broth- diff

sodium thioglycolate reducing agent maintains low oxygen tension

differentiates between organisms with different oxygen tolerances

Thioglycolate broth isolation

To see oxygen metabolism

IUD recovery of Actinomyces

Thioglycolate sites

sterile sites (fluids, tissues)