HEMA 1 HEMATOCRIT AND ESR

Microhematocrit tube dimensions

75mm long

1.2mm bore

Can hold ____ mL of blood

0.05

Plug size

4-6mm

Four layers from top to bottom

1. Fatty

2. Plasma

3. Buffy coat

4. PRBCs

Microhematocrit centrifuge values

5 mins. at 10,000 RPM

Results should agree within _________

1% ( +- 0.01 L/L)

Trapped plasma causes Hct to be?

1-3% increased

False increased Hct

Dehydration

Hemoconcentration

Insufficient centrifugation

Buffy coat inclusion in reading

False decreased Hct

Hemolysis

Improper sealing

Increasing anticoagulant conc.

Introduction of excess IF

Rule of three

Rbc x 3 = Hgb

Hgb x 3 = Hct

Clasically defined as increased hematocrit level above normal

Polycythemia

Elevated total red cell mass (Increased RBC, WBC, Plt)

Absolute polycythemia

Normal total red cell mass, but hematocrit is increased because plasma volume is decreased

Relative polycythemia

Mutation in PV?

JAK2 V617F

Hallmark for PV

Plethora

Treatment of choice for PV

Therapeutic phlebotomy

Westergren anticoagulant

3.8% sodium citrate

Results are determined after _____ and after ______

1 hr., 2 hrs.

Most commonly used method for ESR

Modified westergren

AC for Modified Westergren

EDTA

Modified Westergren diluents

0.85% NaCl

3.8% Sodium Citrate

Modified Westergren dimensions

200mm column

2.55mm bore

Wintrobe results are determined after ____

1 hr.

Wintrobe AC

Double oxalate / EDTA

Left side (red)

0 top, 10 cm bottom for ESR

Right side (white)

10 top, 0 bottom for macrohematocrit

Bench top analyzer designed to measure ESR in 20 samples

VES-MATIC 20

18 degree slant of the tubes with respect to the vertical axis causes?

Acceleration in sedimentation

Modified Westergren ref. range

Males: 0-10 mm/Hr

Females: 0-15 mm/Hr

Wintrobe ref. range

Males: 0-9 mm/hr

Females: 0-20 mm/hr

Proteins: ESR Increased if?

Increased cholesterol, fibrinogen, gamma globulins

Decreased albumin

Proteins: ESR decreased in?

Increased albumin, glucose, bile salts, phospholipids

Decreased fibrinogen, gamma globulins

RBCs: ESR increased in

Anemia, Macrocytosis

RBCs: ESR decreased in?

Polycythemia

Microcytosis

Spherocytosis

Tilt as little as 3^o from the vertical can cause error of up to ______

30%