Histopathologic techniques; Infiltration/Embedding

Temperature that prevents cracking of the tissue block

10C

An ideal embedding medium has to be molten at what temp range

30-60C

Vacuum embedding is wax impregnation under positive or negative atmospheric pressure

Negative

TRUE OR FALSE: Vacuum embedding is suitable for delicate tissues

TRUE

Melting temp of paraffin

65-70C

Paraffin oven should be _ degrees Celsius above melting point of paraffin

2-5C

Paraffin substitute associated with mixture of highly purified wax and synthetic plastic polymers; melting point of 56-57C; more resilient than paraffin; for BONES AND BRAIN

Paraplast

Paraffin substitute associated with less brittle; embedding eyes

Embeddol

Paraffin substitute associated with lower melting point (46-48C); not soluble in water, soluble in 95% ethanol

Ester wax

Paraffin substitute associated with unpleasant annoying OYSTER OR GARLIC TASTE

DMSO

Celloidin method associated with recommended for bones, teeth, large brain sections

Wet method

Celloidin method associated with for eye sections; no 70% alcohol used; Gilson’s mixture

Dry method

Impregnation method associated with rarely used; used if DEHYDRATION is avoided; Gough-Wentworth technique

Gelatin

Material used exclusively as the embedding medium for electron microscopy

Resin

Melted paraffin wax must be at a temperature above how many degrees its melting point

5-10 C

Embedding mold associated with 2 L- shaped pieces of metal

Leuckhart’s

Embedding mold associated with interlocking plates resting on a flat metal base; forms several compartments

Compound embedding unit

Embedding mold associated with plastic walls are peeled off one at a time; no trimming required; placed directly on chuck of microtome

Peel-a-way

Embedding mold associated with made from paper; cheap and allow blocks to be stored without being removed

Paper boat

Embedding mold associated with convenient molds for busy routine labs

Plastic ice tray

Embedding mold associated with embedding fragmented biopsies

Watch glass

Embedding mold associated with useful for small fragments; often necessary to break this mold

Test tubes

Skin biopsies and Nails must be embedded where?

On edge

Trimming may also be referred to as

Facing or rough cutting

Biconcave knives require bigger or smaller clearance angles than wedge-shaped knives

Smaller

Fine trimming requires how many microns

15 microns