Protozoa Identification

Prelims to midterm (SOURCE: CDC) | jcc

Entamoeba histolytica/ Entamoeba dispar

CYST

chromatoid bodies with blunt, rounded ends (arrow)

Entamoeba histolytica/ Entamoeba dispar

CYST

chromatoid body with blunt, rounded ends (arrow).

Entamoeba histolytica/ Entamoeba dispar

CYST

3 nuclei visible, chromatoid body present

Entamoeba histolytica/ Entamoeba dispar

IMMATURE CYST

cyst has large vacuoles and the chromatin around the nucleus is clumpy.

Entamoeba histolytica/ Entamoeba dispar

TROPHOZOITE

DFS, Iodine

Entamoeba histolytica/ Entamoeba dispar

TROPHOZOITE

DFS, Iodine

Entamoeba histolytica

TROPHOZOITE

with ingested erythrocytes (dark inclusions)

Entamoeba histolytica

TROPHOZOITE

with ingested erythrocytes (arrow), DIC microscopy

Entamoeba histolytica

TROPHOZOITE

rectal biopsy

Endolimax nana

CYST

Iodine stained, nuclei visible

Endolimax nana

CYST

unstained, nuclei not visible

Endolimax nana

CYST

trichrome stained

Endolimax nana

CYST

trichrome stained

Endolimax nana

TROPHOZOITE

trichrome

Endolimax nana

TROPHOZOITE

trichrome

Endolimax nana

TROPHOZOITE

trichrome

Entamoeba coli

CYST

unstained. six nuclei visible

Entamoeba coli

CYST

iodine stained, seven nuclei visible

Entamoeba coli

CYST

iodine stained, five nuclei visible

Entamoeba coli

CYST

chromatoidal body visible

Entamoeba coli

IMMATURE CYST

two nuclei visible, large glycogen vacuole

Entamoeba coli

TROPHOZOITE

trichrome

Entamoeba coli

TROPHOZOITE

trichrome

Entamoeba coli

TROPHOZOITE

trichrome

Entamoeba coli

TROPHOZOITE

trichrome

Entamoeba hartmanni

CYST

Iodine

Entamoeba hartmanni

CYST

trichrome

Entamoeba hartmanni

CYST

trichrome

Entamoeba hartmanni

TROPHOZOITE

trichrome

Entamoeba hartmanni

TROPHOZOITE

trichrome

Entamoeba hartmanni

TROPHOZOITE

trichrome

Entamoeba polecki

CYST

Iodine, numerous chromatoid bodies (arrows)

Entamoeba polecki

CYST

trichrome, large nucleus with a pleomorphic karyosome

numerous chromatoid bodies

Entamoeba polecki

TROPHOZOITE

trichrome

Entamoeba polecki

TROPHOZOITE

trichrome

Iodamoeba butschlii

CYST

unstained, glycogen vacuole seen

Iodamoeba butschlii

CYST

Iodine stained, glycogen vacuole seen as a dark-staining mass

Iodamoeba butschlii

CYST

trichrome stained, nucleus and large vacuole seen

Iodamoeba butschlii

TROPHOZOITE

trichrome,

Entamoeba gingivalis

TROPHOZOITE

Entamoeba gingivalis

TROPHOZOITE

Naegleria fowleri

CYST

Naegleria fowleri

AMEBOFLAGELLATE TROPHOZOITE

Naegleria fowleri

TROPHOZOITE

Acanthamoeba spp.

CYST

Acanthamoeba spp.

CYST

brain tissue

Acanthamoeba spp.

TROPHOZOITE

Acanthamoeba spp.

TROPHOZOITE

Acanthamoeba spp.

TROPHOZOITE

corneal scraping

Balantidium coli

CYST

Balantidium coli

TROPHOZOITE

visible cilia

Balantidium coli

TROPHOZOITE

cytostome (black arrow)

Balantidium coli

TROPHOZOITE

cytostome (arrow), cilia (darts)

Giardia duodenalis

CYST

iodine

Giardia duodenalis

CYST

iodine

Giardia duodenalis

CYST

trichrome

Giardia duodenalis

TROPHOZOITE

Iodine, wet mount

Giardia duodenalis

TROPHOZOITE

geimsa

Babesia spp.

TETRAD

Babesia spp.

TETRAD, AMEBOID TROPHOZOITE

Babesia spp.

TETRAD

PAIRS ALIGNED

Babesia spp.

EXTRACELLULAR FORM

indicative of Babesia

Babesia spp.

RING, TETRAD

vacuole

Leishmania spp.

AMASTIGOTES

Leishmania spp.

AMASTIGOTES

Leishmania spp.

PROMASTIGOTES

Trypanosoma cruzi

AMASTIGOTES

heart tissue

Trypanosoma cruzi

TRYPOMASTIGOTE

CSF sample

Trypanosoma cruzi

EPIMASTIGOTE

kinetoplast anterior to the nucleus

Trypanosoma cruzi

TRYPOMASTIGOTE

Trypanosoma cruzi

TRYPOMASTIGOTE

typical C-shape of the trypomastigote that characterizes T. cruzi in fixed blood smears

Trypanosoma brucei subsp.

TRYPOMASTIGOTE

Trypanosoma brucei subsp.

TRYPOMASTIGOTE

Trypanosoma brucei subsp.

TRYPOMASTIGOTE

Trypanosoma brucei subsp.

TRYPOMASTIGOTE

• beginning to divide

• dividing forms are seen in African trypanosomes, but not in American trypanosomes

Plasmodium falciparum

RINGS

Plasmodium falciparum

RINGS

exhibiting Maurer's clefts

Plasmodium falciparum

TROPHOZOITES

Plasmodium falciparum

TROPHOZOITES

Plasmodium falciparum

GAMETOCYTES

Plasmodium falciparum

GAMETOCYTES

Plasmodium falciparum

GAMETOCYTES

The gametocyte in the upper right is undergoing exflagellation, a process that normally occurs in the mid-gut of the mosquito host.

Plasmodium falciparum

SCHIZONTS

Trophozoites are also seen

Plasmodium falciparum

SCHIZONTS

Plasmodium knowlesi

RINGS

RBC multiply-infected

Plasmodium knowlesi

RINGS

RBC multiply-infected

Plasmodium knowlesi

TROPHOZOITES

Plasmodium knowlesi

TROPHOZOITES, RING

Plasmodium knowlesi

GAMETOCYTES

Plasmodium knowlesi

GAMETOCYTES

Plasmodium knowlesi

SCHIZONTS

Plasmodium knowlesi

SCHIZONTS

Plasmodium malariae

RING FORM TROPHOZOITE

Birds-eye

Plasmodium malariae

RING FORM TROPHOZOITE

Plasmodium malariae

DEVELOPING TROPHOZOITE

Plasmodium malariae

MATURE TROPHOZOITE/ BAND FORM

Plasmodium malariae

MATURE TROPHOZOITE/ BASKET FORM

Plasmodium malariae

GAMETOCYTES

Plasmodium malariae

GAMETOCYTES

Plasmodium malariae

SCHIZONTS

rosette cluster