recombinant plasmid

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6 Terms

1
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Isolation

Extract the gene of interest and a plasmid from bacterial cells

2
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Cutting dna

use restriciton enzymes to cut the gene and plasmid at specific recognition sites creating sticky or blunt ends

3
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ligation

join the gene and plasmid using dna ligase forming a recombinant plasmid

4
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transformation

introduce the plasmid into the host cell through heat shock or electrophorchion

5
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selection

identify successful transformation using selectibe markers

6
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cloning and expressions

the host cells replicate passing the recombinant plasmid along potentially expressing desired protein