recombinant plasmid
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Last updated 11:16 AM on 3/13/25
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6 Terms
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Isolation
Extract the gene of interest and a plasmid from bacterial cells
2
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Cutting dna
use restriciton enzymes to cut the gene and plasmid at specific recognition sites creating sticky or blunt ends
3
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ligation
join the gene and plasmid using dna ligase forming a recombinant plasmid
4
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transformation
introduce the plasmid into the host cell through heat shock or electrophorchion
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selection
identify successful transformation using selectibe markers
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cloning and expressions
the host cells replicate passing the recombinant plasmid along potentially expressing desired protein
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Flashcards (23)