Refinement and Details of Replication

Function is supercoiling and untangling

DNA topoisomerase I and II

Function is unzipping the DNA Helix

Helicase

Function is Adding bases to DNA chain; proofreading the chain for mistakes

DNA polymerase III

Function is removing primer, closing gaps, repairing mismatches

DNA polymerase I

Function is final binding of nicks in DNA during synthesis and repair

DNA Ligase

Function is synthesizing an RNA primer

Primase

Function is helping to untangle DNA supercoils

Gyrase

What keeps the two strands apart during dna replication

single strand binding proteins

What end can DNA Polymerase III add nucleotides to CONTINUOUSLY?

3’ end of daughter strand

If the parent is going in the 3’ to 5’ (in relation to the fork) direction how must the daughter strand be synthesized?

5’ to 3’

The daughter strand being synthesize grows continuously towards the replication fork, this strand is called the ______ daughter strand

Leading

How is DNA polymerase oriented for DNA synthesis (direction of NEW molecule in relation to fork)

5’ to 3’

If the parental strand is going in a 5’ to 3’ direction in relation to the replication fork, the daughter proceed in what direction

3’ to 5’

In what direction is the lagging strand synthesized

Away from the fork (5’ to 3’)

What is required on EACH fragment of DNA synthesized on the LAGGING strand (away from fork)

RNA primers

What enzyme removes the RNA primers from the beginning of each fragment and at the end of replication?

DNA polymerase I

What enzyme joins the Okazaki fragments?

DNA Ligase