Test for biomolecules
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11 Terms
Lobry de Bruyn van Eikenstein rearrangement
Conversion of fructose into mixture of glucose and mannose under basic condition
Glucose has what group
-CHO
Fructose as what group
-C=O
Why does fructose reduce fehling solution eventho it doesnt have -CHO group
Because under basic condition of the reagent, fructose undergoes isomerization and converts into a mixture of glucose and mannose - both of which have -CHO group
Molisch Test
Used for detection of all types of carbohydrates
Molisch reagent
1% alcoholic solution of alpha-naphthol
What are the steps for Molisch test
Molisch regent added to aq sol. of carbohydrates
followed by conc. H2SO4 along sides of test tube
Result of Molisch test
A violet ring is formed at the junction of the two layers
Biuret test
used to test for proteins
What is used in biuret test
An alkaline solution of protein is treated with few drops of 1% CuSO4 solution
Result of Biuret test
Produces a violet colour due to formation of coodination complex of Cu+2 with -C=O and
-NH- groups of peptide linkages
Note 
Flashcards (99)