microbe- Moraxella, Brucella, Campylobacter

infectious bovine keratoconjunctivitis (pink eye)

what is the disease caused by Moraxella bovis?

fimbriae that adhere to the cornea

hemolysin- damages cornea and neutrophil membrane, which releases hydrolytic enzymes

fibrinolysin

phosphate

hyaluronidase

aminopeptidase

what pathogenic factors does Moraxella have?

Moraxella bovis

which bacteria causes infectious bovine keratoconjunctivitis?

hemolysin

what important enzyme does Moraxella bovis produce that causes damage to the cornea and neutrophil membranes, causing the release of hydrolytic enzymes?

it is commensal on mucous membranes

also transmitted by DC, aerosols, flies

how is Moraxella bovis transmitted?

ocular irritants

vitamin A deficiency

youth

lysozome deficiency in lacrimal secretions

what factors predispose a carrier animal to infectious bovine keratoconjunctivitis?

edema, vascularization, and granulation tissue formation from the ulcer in the eye

the disease is called infectious bovine keratoconjunctivitis (pink eye)

what are the effects of Moraxella bovis on the eye?

Moraxella bovis

infectious bovine keratoconjunctivitis (pink eye)

what bacteria causes this? what is the name of this disease?

Moraxella bovis

this appearance is characteristic of what bacteria?

yes

does Moraxella bovis need O2 to grow?

no

is Moraxella bovis motile?

+,+

Moraxella bovis is catalase _____ and oxidase _____

yes

is Moraxella bovis haemolytic?

no

can Moraxella bovis grow on macconkey?

lacrimal secretions

they need to be placed in water/saline solution because this bacteria is very susceptible to desiccation

what is the best sample of Moraxella bovis?

Brucella

which bacteria needs erythritol to grow?

B. abortus- cattle

B. melitensis- goats

B. suis- pigs

B. canis- dogs

what are the 4 important species of Brucella and what animals do they infect?

YES

does Brucella need to be inside of a cell?

no

does Moraxella need to be inside of a cell?

reproductive organs in sexually mature animals

what organs does Brucella target?

ingestion or direct contact with organisms from aborted fetuses and post abortion/post calving discharge

during copulation (semen, vaginal discharge)

how is Brucella transmitted?

no

is Moraxella bovis zoonotic?

yes

is Brucella zoonotic?

Less virulent: no LPS, rough colonies, easily eliminated

More virulent: LPS in outer membrane, smooth colonies, persists and multiplies

what are the 2 types of strains of Brucella?

rough

a less virulent strain of Brucella produces _____ colonies

smooth

a more virulent strain of Brucella produces _____ colonies

if it is her first pregnancy, she will have an abortion

if it is not her first pregnancy, she will have a high amount of shedding at birth

if a pregnant female gets infected by Brucella, what happens?

the bacteria goes to lymphatic tissues and there are no clinical signs

what happens if a non-pregnant female gets infected by Brucella?

orchitis, epididymitis, infertility, shedding in semen

what happens to a bull that gets Brucella?

Brucellosis

Brucella

these reproductive issues are produced in what zoonotic disease? what bacteria causes it?

reproductive issues

can also cause arthritis if it goes to joints

what issues does Brucella cause in an infected animal?

no

can Brucella grow without O2?

+,-

Brucella is catalase _____, oxidase _____

no

is Brucella motile?

red, because it is +

how does Brucella appear with the MZN stain?

Brucella

which bacteria appears like this with the MZN stain?

no

is Brucella hemolytic?

Brucella

what bacteria can grow on serum agar?

abortion material

udder secretions (milk)

post mortem tissues

what samples are appropriate for diagnosing an animal with Brucella?

Brucella

adding a drop of reagent with Brucella antigens to a milk sample

what bacteria is the milk ring test used to detect? how is it performed?

middle tube

Brucella

which tube shows a positive result for the milk ring test? which bacteria does it have?

Brucella

what bacteria is the Rose-bengal plate test used to detect?

on the right- agglutinated

Brucella

which Rose-bengal test is positive? which bacteria is it positive for?

Campylobacter

what bacteria causes the #1 disease caused by food contamination?

yes

is Campylobacter zoonotic?

C. fetus fetus

C. fetus venerealis

C. jejuni

C. coli

what are the four genera of Campylobacter?

reproductive tract (cattle)

where is Campylobacter fetus venerealis commensal in the body?

ruminants

what species does Campylobacter fetus fetus affect?

cattle

what species does Campylobacter fetus venerealis affect?

intestinal tract (ruminants)

where is Campylobacter fetus fetus commensal in the body?

intestinal tract of mammals and birds

where is Campylobacter jejuni commensal in the body? of what animals?

pigs; intestinal tract

what animals does Campylobacter coli infect? where in the body is it present?

Campylobacter fetus fetus

what species of Campylobacter is normally present in the intestinal tract of ruminants but produces a reproductive infection?

Campylobacter fetus venerealis

Campylobacter fetus fetus

what species of Campylobacter produce reproductive issues?

Campylobacter jejuni

Campylobacter coli

what species of Campylobacter produce enteritis?

Campylobacter

what bacteria is this shape?

yes

is Campylobacter motile?

no

is Campylobacter hemolytic?

Campylobacter

what bacteria can grow on mCCDA and CASA selective mediums?

intestinal tract; pigs; enteritis

Campylobacter coli is commensal in the ______ of ______ and causes _____

intestinal tract; mammals and birds; enteritis

Campylobacter jejuni is commensal in the ______ of ______ and causes _____

yes, it is in fact the #1 producer of food contamination disease

can Campylobacter infect humans?