Transcriptional regulation

What does the CAP site stand for

catabolic activator protein

what does the Lac Z gene encode

beta galactosidase

What does the Lac Y gene encode

lactose permease

What does the Lac A gene encode

thiogalactoside transacetylase

What does Lac I gene encode

repressor

what molecule binds to repressor when lactose concentration is high

allolactose

what do coactivators bind to

activators

what are the two domains of activators

activation, DNA-binding

What are the two main epigenetic mechanisms

DNA methylation, histone modification

what sites are methylated in DNA

CG sites

does methylation of gene body lead to increase or decreases in transcription

increase

does hypermethylation of promoter sequences lead to gene activation or silencing

silencing

does hypomethylation of promoter sequences lead to gene activation or silencing

activation

what enzyme helps retain methylation during DNA replication

DNA Methyltransferase 1

is the active IGF allele passed from the mother or father

father

is the active H19 allele passed from the mother or father

mother

is IGF2 a positive or negative growth regulator

positive

is H19 a positive or negative growth regulator

negative

what is the name of the disease where IGF2 is overexpressed

Beckwith-Weidemann syndrome

what is the name of the disease where H19 is overexpressed

Silver russell syndrome

what are the two main types of histone modifications

acetylation, methylation

which enzymes are the writers of histone acetylation

acetyltransferases

which enzymes are the erasers of histone acetylation

deacetylases

what type of charge do histone tails have

positive

with what domains do regulatory proteins bind to acetylated histones

bromodomains

what are common histone methylations

H3K4, H3K9, H3K27

what methylation activates enhancer regions

H3K4me1

what methylation activates promoter regions

H3K4me3