Micro lab exam 3

Durham tube results

positive is e coli with yellow with gas bubble, produced acid, negative is A faecalis, with red

Durham tube media

phenol red, glucose and a small inverted tube

Mixed acid methyl red results

well color changes red for positive e coli and acid formic hydrogenylase produced, negative no color change bacillus subtilis

mixed acid methyl red media

methyl red, glucose broth

Butanediol Vosges-Proskauer results

dark red well is positive B. subtilis for acetoin, butanediol production, e coli is negative

Butanediol Vosges-Proskauer media

alpha naphthol 40% KOH

catalase production results

plate bubbles positive for catalase production s epidermidis, e faecalis is negative with no gas

catalase production media

hydrogen peroxide

oxidase production results

plate turns blue for positive cytochrome oxidase production from A faecalis, while E faecalis is negative

oxidase production media

tsa+, difco

starch hydrolysis results

clear zone around iodine is positive b subtilis of amylase, and negative is not clear e coli

starch hydrolysis media

grams iodine, tsa

Casein hydrolysis results

clear zone around b subtilis positive for caseinase production, and a faecalis is negative with no clearing

casein hydrolysis media

casein agar, skim milk

fat hydrolysis results

pink area around growth is positive for lipase production s epidermidis, negative for a faecalis

fat hydrolysis media

peanut oil agar, neutral red indicator

tryptophan hydrolysis indole results

red ring around yellow broth is positive for typtophanase e coli, all yellow is negative E aerogenes

tryptophan hydrolysis indole media

tryphone broth kovacs reagent

urea hydrolysis results

pink color change is positive for urease production p vulgaris, no color change is negative e coli

urea hydrolysis media

urea agar, yeast, phenol red

gelatin hydrolysis results

a clear zone around growth is positive for gelatinase production P fluorescens, negative is cloudy e coli

gelatin hydrolysis media

gelatin developer

hydrogen sulfide production results

black precipitation is positive for production of hydrogen sulfide of p vulgaris, clear is negative of e coli

hydrogen sulfide production media

hydrolyzed casein ferrous salts

citrate utilization results

blue slant is positive for citrate utilization and pH increase e aerogenes, green is negative e coli

citrate utilization media

bromothymol blue indicator

phenylalanine deamination results

green color is positive for phenylalanine deaminase p vulgaris, and clear in negative e coli

phenylalanine deamination media

10% ferric chloride

glucose oxidation results

yellow at top, green at bottom positive for carbonic acid production p fluorescens, yellow completely is e coli

glucose oxidation media

OF medium pH indicator

nitrate reduction results

dark blue well is positive for nitrate reduction NO2 present

Mannitol salts agar plate

from pink to yellow for fermentation of mannitol with acid production

Durham tubes?

right is positive

mixed acid(methyl red)?

right is positive

butanediol vosges proskauer?

well on right is positive

catalase production?

bubbling

oxidase production?

dark blue

nitrate reduction?

left is positive

starch hydrolysis

left is positive

casein hydrolysis?

left is positive

fat hydrolysis?

left is positive

tryptophan hydrolysis

left is positive

urea hydolysis?

left is positive

gelatin hydrolysis

left is positive

hydrogen sulfide production?

left is positive

citrate utilization

left is positive

phenylalanine deamination

right is positive

mannitol salts agar plate

blood agar plate

staphylococcus agglutination

neutrophil

basophil

eosinophil

lymphocyte

monocyte

transformation

genes are transferred as naked DNA

competent

when cells are in a state when they can take up DNA

plasmid

pGLO, contains GFP green fluorescent protein, also carries protein that enables antibiotic resistance.

antimicrobic

compounds that kill/inhibit organisms

antibiotic

antimicrobial naturally produced by organisms to kill/inhibit other organisms

agar used on antimicrobial sensitivity testing?

Mueller Hinton agar

antiseptics

limit microbial growth on living tissue that prevent infection like iodine

disinfectant

chemical agents used on inanimate objects like bleach