Lab #6 - Streak Plating and Viable Plate Count

These flashcards cover key vocabulary terms and their definitions related to streak plating and viable plate count in microbiology.

Streak Plate Technique

A method used in microbiology to isolate a pure strain from a single species of microorganism.

Aseptic technique

Practices used to prevent contamination by pathogens or unwanted microorganisms.

Binary fission

The process by which a cell divides into two identical daughter cells.

Viable plate count

A method used to estimate the number of living bacteria in a sample by counting isolated colonies.

Pipette

A laboratory tool used to transport a measured volume of liquid.

Dilution series

A series of solutions with decreasing concentrations used to obtain a range of bacterial concentrations.

Colony

A visible cluster of microorganisms that originates from a single bacterium.

LB agar plates

Lysogeny broth agar plates used for growing bacteria.

Spread-plate technique

A method of plating bacteria in which a small volume is spread across the surface of an agar plate.

Contamination

The introduction of unwanted microorganisms into a culture.

Blow out pipette

A type of pipette designed to deliver a specific volume of liquid and ensure complete discharge by blowing out the remaining liquid.

to delivery pipette

A type of pipette designed to accurately deliver a specific volume of liquid, typically using a blowout feature to ensure complete transfer.

s

suck

a

aspirate

e

empty

How would you know if your plate is contaminated?

Cells would be different colors, shapes, sizes etc.