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where
s-phase
is replication conservative or semi-conservative
semi-conservative
semi-conservative rep
one daughter strand + one parent strand
dna polymerase
synthesis of polynucleotide strand
topoisomerase
decoiling
helicase
unwinding
primase
attaches rna primer for dna strand synthesis
ligase
phosphodiester bond formation
telomerase
replicates dna at end of chromosomes
duplication
prevents mutations
stages
initiation, elongation, termination
initiation
unwinding the DNA double helix with helicase and assembling the replisome to start synthesis
elongation
new DNA strands synthesized by DNA polymerase, adding complementary nucleotides to existing template strands
termination
replication forks meet, synthesis completes, the machinery disassembles, and intertwined daughter DNA molecules are separated
is RNA polymerase involved in transcription
yes
does rna poly require transcription factors
yes- for correct binding to start site
rna poly bases
AUGC
rna poly reads in
3’ to 5’
protein kinase
requires ATP for phosphate