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Vocabulary flashcards covering key terms and concepts from the microbiology notes (Ch. 1).
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Microbiology
The study of microorganisms that are invisible to the naked eye.
Bacteria
Single-celled prokaryotes; some species cause disease.
Fungi
Eukaryotic organisms including yeasts and molds.
Prions
Infectious protein particles lacking nucleic acids.
Protists
A diverse group of mostly unicellular eukaryotic organisms.
Helminths
Parasitic worms (flatworms and roundworms).
Viruses
Non-cellular infectious agents that require a host to replicate.
Archaea
Single-celled prokaryotes distinct from bacteria; often in extreme environments.
Biopharmaceutical production
Use of microbes to produce drugs, vaccines, enzymes; fermentation-based manufacturing.
Fermentation
Microbial process to produce foods and beverages; used in industry.
Bioremediation
Use of microorganisms to degrade environmental pollutants and clean up hazards.
Virology
Study of viruses.
Mycology
Study of fungi.
Bacteriology
Study of bacteria.
Parasitology
Study of parasites.
Pathogens
Disease-causing microbes.
Opportunistic pathogen
Microbes that cause disease only in immunocompromised hosts.
Golden Age of Microbiology
Period 1850–1920 with major innovations and techniques still used today.
Spontaneous generation
Idea that life can arise from non-living materials.
Biogenesis
Idea that life arises from existing life.
Louis Pasteur
Disproved spontaneous generation; contributed to germ theory and pasteurization.
Koch's postulates
Four criteria linking a microbe to a disease: (1) present in every case, (2) isolated and grown in pure culture, (3) causes disease when inoculated, (4) re-isolated from diseased host.
Binomial nomenclature
System of naming organisms with two names: Genus and species.
Normal microbiota
Microbiota that normally reside on/in the body; train immune system, produce vitamins, aid digestion, may affect mood/brain.
Transient microbiota
Temporary microbes that reside on the body and are not permanent residents.
Growth media
Nutrient mixtures that support the growth of bacteria in the lab.
Colony
A visible group of cells originating from a single parent cell.
Pure culture
A culture containing only one microbial type.
Aseptic technique
Procedures that limit contamination of cultures and environments.
Mixed culture
Cultures containing more than one type of microbe; shows different colonies.
Smear
Thin film of sample spread on a slide for staining.
Heat fixation
Fixing the smear to the slide by passing through a flame.
Staining
Applying dyes to visualize cells under a microscope.
Endospore stain
Structural stain used to visualize endospores.
Flagella stain
Structural stain used to visualize flagella.
Gram stain
Differential stain classifying bacteria as Gram-positive or Gram-negative.
Acid-fast stain
Differential stain for Mycobacteria; resists decolorization.
Primary stain
Initial dye in a differential stain.
Mordant
Substance that fixes the dye to cells in a differential stain.
Decolorizer
Agent that removes stain from some cells in a differential stain.
Counterstain
Second dye providing contrast in a differential stain.
Crystal Violet
Primary stain in Gram staining.
Gram's Iodine
Mordant in Gram staining.
Ethanol/Acetone
Decolorizer in Gram staining.
Safranin
Counterstain in Gram staining.
Compound light microscope
The most common microscope used in labs.
Alexander Fleming
Discovered penicillin.
Robert Hooke
First to observe and publish descriptions of cells.
Edward Jenner
First to vaccinate against smallpox.
Robert Koch
First to prove that microbes cause diseases.
Carl Linnaeus
Developed a naming system for organisms (binomial nomenclature).
Joseph Lister
First to use aseptic techniques in surgery.
Florence Nightingale
Established aseptic practices in nursing.
Julius Petri
Developed the Petri dish for culturing bacteria.
Ignaz Semmelweis
First to implement handwashing to prevent childbed fever.
Antonie van Leeuwenhoek
First to observe bacteria; father of microbiology.