lab exam

Lipase is positive?

Right side (Clearing in agar around growth)

Decarboxylase is present

Purple

Decarboxylase is not present

Yellow

Catalase is positive

Bubbles

Starch is positive

Had a cleaning of medium (a straight line down the middle)

Acid fast

Presence of mycolic acids

Citrate Utilization Test: Blue

Citrate is utilized (+)

Citrate utilization test: No color change, no growth

Citrate is utilized (citrate permeate is present)

Citrate utilization test: No color change no; growth

Citrate is not utilized (citrate permease is absent or not detectable)

Phenol Red Fermentation; yellow broth, bubble in tube

Fermentation of the specific carbohydrate with acid and gas products

Phenol Red Fermentation; yellow broth, no bubble in tube

Fermentation of the specific carbohydrate with acid end products; no gas product, or gas produced in undetectable amounts

Phenol Red Fermentation; Pink broth, no bubble

Degradation of peptone, alkaline end products

Methyl red test results: Red

Organism performs a mixed acid fermentation

Methyl red test results: Orange or pink

Organism does not perform a mixed acid fermentation or then end products are not made in detect-able amounts

Votes-Proskauer Test: Red or Pink

Organism performed a 2,3 butanaedoil fermentation (action produced)

Votes-Proskauer Test: No color change

Organism does not perform a 2,3-butanediol fermentation

Sulfur reduction test: Black in the medium

Sulfur reduction (H2S production); organism either respires anaerobically with sulfate as the final electron acceptor or hydrolysis the amino acid cysteine (+)

Sulfur reduction test: No black in the medium

Sulfur is not reduced, or is not reduced in detectable amounts (-)

Insole production test: Red in the alcohol layer of Kovac’s regent

Organism produces tryotophanase and hydrolyzes tryptophan into indlolr and pyrubate (+)

Insole production test: Reagent color is unchanged

Tryptophanase is absent and indoor is not produced or is produced but not in recreate amounts (-)

Motility Test: Growth radiating outward from stab line

Motility (+)

Motility test: No radiating growth

Nonmotile (-)

Motility test: Blackened medium

Motility not determined

Lipase is absent

No clearing in agar around the growth

Gelatin Hydrolysis Test: Positive

Gelatinase is liquid. Gelatinase is present

Gelatin Hydrolysis Test: Negative

Gelatinase is sold. Gelatinnase is absent or nor detectable