E. coli: Identification and susceptibility

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5 Terms

1
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Old Test of STEC (5)

  1. Culture on SMAC plate

  2. Serotype for O157

  3. Subculture on blood agar

  4. Serotype for H7

  5. Problem: Other somatic types cause STEC

2
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Tests now for STEC

Shiga toxin testing right off of stool

3
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The different biochemical reaction between E.coli and H. coli H7:O157

  1. E. Coli: Sorbitol +

  2. E. Coli H7:O157: Sorbitol -

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CLSI for E. coli (2)

  1. Ampicillin

  2. First gen cephems

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Why is it controversial to use antibodies for EHEC?

  1. Antibiotics can do something to the cells to make EHEC worse

  2. Stay away from antibiotics UNLESS in bloodstream

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