Quiz 6 - Bacterial Enumeration

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9 Terms

1
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What does CFU stand for?

Colony-forming unit

2
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What is the purpose of serial dilutions?

decrease the concentration of bacteria for easier counting

3
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What is the countable range for bacterial enumeration?

30-300 colonies

4
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What does the spectrophotometer measure in bacterial enumeration?

optical density/turbidity

5
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What are some examples of DIRECT bacterial counting methods?

  1. microscope

  2. hemocytometer

6
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What is an example of a VIABLE bacterial counting method?

Plate count method

7
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What is an example of an INDIRECT bacterial counting method?

turbidity via spectrophotometer

8
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What is the best method for visualizing both live and dead bacterial cells?

Microscope count using a hemocytometer with cell staining

9
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Why is measuring the turbidity of a sample no a direct measure of viable cells?

Measures the total cell mass, including dead cells