P1: Introduction

What are examples of diagnostic methods in parasitology?

coprological, urine, skin, haematological, histopathological, immunological, molecular biological

Which is the most common laboratory diagnostic method?

coprological (faecal) examination

When should faecal samples be used after collection?

within a few hours (or refrigerated)

How can helminth eggs be preserved in a faecal sample?

equal volume of 5-10% buffered formalin

Why is faecal examination the most common laboratory diagnostic procedure?

it can reveal the presence of parasites in several body systems

What are the requirements for collecting faecal samples?

plastic gloves, wooden spatulas, containers, formol, means to identify sample

How much faeces should be collected for a sample from cattle?

20-30 g

How much faeces should be collected for a sample from equids?

20-30 g

How much faeces should be collected for a sample from sheep?

10-20 g

How much faeces should be collected for a sample from swine?

10-20 g

How much faeces should be collected for a sample from carnivores?

3-10 g

How much faeces should be collected for a sample from rabbits?

3-10 g

How much faeces should be collected for a sample from birds?

3 g or 3 sequential defecations

What is the necessary data needed to identify a sample?

animal species, animal owner, date, address

Where should blood be collected from for diagnostic procedures?

peripheral veins

Why should blood be collected from peripheral veins for diagnostic procedures?

skin is soft and connects with ectoparasites, which release other parasites to the blood;
blood pressure is lower, so better habitation for parasites

How should samples be taken for sampling of ectoparasites?

scratch by scalp, pulling out hairs

What is required when sending samples?

distinct and indelible mark;
accompanying note (anamnesis, type of examination, name and address, species of animal, sex, age)