Bio 120 Practical

Parts of onion cell

Nucleus, nucleolus, cell wall, central vacuole, cytoplasmic strand, cellular granules

Parts of green pepper cell

cell wall, chloroplasts, central vacuole, nucleus, nucleolus

Parts of red pepper cell

cell wall, plasmodesmata, chromoplasts, central vacuole, nucleus

Parts of banana cell

cell wall, starch grains, cytoplasm, striations

Parts of cheek cell

plasma membrane, cytoplasm, nucleus

How to set up cheek cell slide

Drop or two of methylene blue stain on slide

scrape inside of cheek, immediately transfer to stain and stir

apply coverslip to slide

How to set up slide for banana cell

drop of water on slide

on toothpick scrape banana tissue

mix with water

apply coverslip to slide.

How to set up slide for red pepper cell

drop of water on slide

using fine forceps, carefully peel skin from surface of pepper

apply cover slip

How to set up slide for green pepper cell

drop of water to slide

using razor blade, make series of thin cross section of a piece of green pepper

apply cover slip

How to set up slide for onion cell

drop of water to centre of slide

using fine forceps, carefully peel clear skin and place in water

apply coverslip to slide.

Ensure light intensity control is alway set at what

7

What should the lens start on

4x

Where should everything start?

stage down, condenser all the way up

When is iris diaphragm open?

all the way to the left

How to only see one field of view

move optic lenses

Where do you look to position specimen in whole of stage?

to the side looking in

Order of focusing

1. right with knob on side

2. left with optic lens

How do you set condenser and when?

After each eye is focused

Place pencil on light source shadow should be fuzzy

Use focus condenser knob until shadow is sharp

(condnser knob is left of stage in front of focus knob)

Do you redo the light intensity

Every switch of magnification