Lecture 8: Enzymes Kinetic

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Quiz 3

Last updated 9:26 PM on 5/5/25
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phases in enzyme catalysis

pre-steady state → steady state

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pre-steady state

initial transient period during which ES builds up

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steady state

period during which [ES] and other intermediates remain constant

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What assay experiment can be done to determine the relationship between initial velocities and substrate?

  • mix enzyme and substrate

  • record rate of substrate disappearance and/or product formation as a function of time (the velocity of reaction)

  • repeat with a higher [s]

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