Lecture 8: Enzymes Kinetic
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Quiz 3
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5 Terms
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phases in enzyme catalysis
pre-steady state → steady state
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pre-steady state
initial transient period during which ES builds up
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steady state
period during which [ES] and other intermediates remain constant
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What assay experiment can be done to determine the relationship between initial velocities and substrate?
mix enzyme and substrate
record rate of substrate disappearance and/or product formation as a function of time (the velocity of reaction)
repeat with a higher [s]
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Note 
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