DNA Replication and its Applications

Flashcards summarizing key concepts and answers regarding DNA replication, PCR, and DNA sequencing based on lecture notes.

Why is helicase not needed in PCR?

Helicase separates the two strands of DNA, but PCR uses heat to separate the strands instead.

What role does primase play in DNA replication?

Primase synthesizes primers during DNA replication.

Why is primase not needed in PCR?

Primase is not needed in PCR because DNA primers are supplied in the reaction.

What method would you use to identify a point mutation in a DNA sequence?

I would use DNA sequencing because it provides the sequence of DNA; PCR only produces copies.

What effect does forgetting to add fluorescently labeled ddCTP have on sequencing?

The sequencing reaction will not terminate at C, preventing the identification of C base locations.

What technique would you use to determine the species of a mystery fish?

DNA sequencing, because it provides the sequence information needed to compare with known fish DNA sequences.

What is the best technique for sequencing an entire genome?

Next generation sequencing is the best technique for sequencing an entire genome.