CP 06 - Prepare and stain a root tip squash to observe the stages of mitosis.

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Equipment list

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Description and Tags

4 Terms

1

Equipment list

  • Root

  • Scalpel

  • Ruler

  • Boiling tube

  • Hydrochloric acid

  • 25 cm³ measuring cylinder

  • Pipette

  • Distilled water

  • Paper towels

  • Microscopic slide

  • Cover slip

  • Mounted needle

  • Stain

  • Optical microscope

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2

Method

  1. Use a scalpel to measure and cut the end 1 cm of the root.

  2. Measure out 10 cm³ of 1 mol/dm³ hydrochloric acid into a boiling tube and place it in a water bath at 60°C.

  3. Transfer the root tip section into the boiling tube and leave it for 5 minutes.

  4. Remove the tip and use a pipette filled with distilled water to rinse it. Leave it to air dry on a paper towel.

  5. Now cut the end 5mm of the root tip and place this on a microscopic slide. Use a mounted needle to spread the specimen out forming a thin layer that will allow light to pass through when observing it under a light microscope.

  6. Use a pipette to apply a couple drops of stain to the specimen, the stain binds to chromosomes making them easier to see, use Toluidine Blue O or Feulgen Stain.

  7. Finally, place a cover slip on top of the specimen on the microscopic slide. Press down firmly , further spreading and thinning viewed under an optical microscope.

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3

Mitotic Index

Cells undergoing mitosis/total cells

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4

boo

bs

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