BLOODBANK 1

POPE INNOCENT VII

First blood transfusion

BRAXTON HICKS

Recommended **Sodium Phosphaate** as blood preservative

KARL LANDSTEINER

Discover the **ABO** blood group system

HUSTIN 1914

Uses **Sodium Citrate** as an anticoagulant

LEWISOHN 1915

Determined the minimum amt of Citrate needed for blood preservative

ROUS & TURNER 1916

Introduced the **Citrate Dextrose preservative**

DR. CHARLES DREW

Director of first **American Red Cross** blood bank

LOUITIT & MOLLISON

Introduced the **ACD** preservative

GIBSON 1957

Introduced **Citrate Phosphate Dextrose (CPD**) preservative

SEGREGATION

**1ST MENDELS LAW**

ASSORTMENT

2ND Mendels Law

DOMINANCE

3rd Mendels Law

46

__Total number__ of **chromosomes** on single healthy human

22

Number of **AUTOSOMES** a single human will inherit form each parent

44

__Total no. of__ **Autosomes** a single human had

CODOMINANT

Most of the Ag in the various blood group system generally follow straightforward inheritance patterns, usually of a nature.

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The inheritance of most BGS

AMORPH

A gene that does nnot appear to produce a detectable Ag

SILENT GENE such as Jk,Lu, O, d.

1ST MENDEL LAW

Law of INDEPENDENT or RANDOM SEGREGATION

States that the parental genes must separate randomly and equally into ggametes during meiosis so there is an equal chance to the offspring inheriting either allele.

No allele is favored or has a adv over another.

2ND MENDEL LAW

Law of independent ASSORTMENT

State that the genes for diff traits are inherited separately form each other.

This allows for all possible combinations of genes t occur in the offspring.

3RD MENDEL LAW

Law of DOMINANCE

POINT OF MUTATION

Simplest type of mutation

Only ONE nucelotide in the DNA sequence is change.

Include Substituuttions,insertions and deletions..

MISSENSE POINT MUTATION

Type of mutation that can have a DELETERIOUS EFFECT on peptide sequence.

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NONSENSE POINT MUTATION

VERY SPECIFIC type of SERIOUS mutation, results when a point change in one of thee nucleotides of a DNA SEQUENCE causes one of the THREE POSSIBLE STOP CODONS to be formed.

TRANSLOCATION

Cut off/ break off and transfer of portion of one chromosome to its allele

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KARYOTYPE

PHOTOMICROGRAPH of a single cell in the metaphase stage of mitosis that is arrangedd to show the chromosomes in descending order of isze.

BLOOD BANKING LAW OF 1956

RA: 1517

DATE: JUNE 16, 1956

SECTIONS: 7

PRES: MAGSAYSAY

NATIONAL BLOOD SERVICES ACT OF 1994

RA: 7719

DATE: MAY 5, 1994

SECTIONS: 15

PRES: RAMOS

CITRATE

Acts as anticoagulant

Chelates Ca

DEXTROSE

Provides energy or glucose to RBC

**SUBSTRATE TO ATP**

CITRIC ACID

Lowers pH thus prevent CARAMELIZATION

MONOBASIC NaPO4

Maintains pH during storage;

Maintenance of adequate levels of 2,3 DPG Inc. ATP lvl

ADENINE

Production of ATP

Improve RBC survival form 21-35 days

LIGHT SPIN > HEAVY SPIN

Preparation for random Donor Plts

5\.5 x 10(10) PLTS\`

Amt of Random Donor Plts

50-75ml

Vol of plasma in Random Donor Plts

PLTPHERESIS

Preparation for Single Donor Plt

3\.0 x 10(11) PLTS

Amt of Single Donor Plts

300ml

Vol. of plasma in Single Donor Plt

6\.2

pH of RDP & SDP

20-24’C W/ AGITATION

Storage of RDP & SDP

5DAYS

Shelf life of RDP & SDP

FFP

**Correct** multiple coagulation factor deficiency

**Replace** isolated factor deficiencies when specifiic component is not available

Reverse effect of WARFARIN anticoagulant drug

1yr

Shelf life of FFP @ -18’C

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7yrs

Shelf life of FFP @ -65’C

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\-18’C or -65’C

Storage of FFP

CYROPRECIPITATE

For tx of FIBRINOGEN def, HEEMOPHILIA A, vWD andd factor XIII def.

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18’C

storage of CRYOPPT

shelf life; 1yr

21 DAYS

BLD COMPONENT SHELF LIFE:

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Acid- citatee dextrose (ACD)

Citrate PO4 Dextrose (CPD)

CItrate PO4 Double Dextrose (CP2D)

35 DAYS

BLD COMPONENT SHELF LIFE:

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Citrate PO4 Adenine (CPDA-1)

42 DAYSS

BLD COMPONENT SHELF LIFE:

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CPDA-2

ADSOL

NUTRICEL

OPTISOL

SOLX

24 HRS

BLD COMPONENT SHELF LIFE:

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OPEN SYSTEM

WASHED RBC

2 YRS

BLD COMPONENT SHELF LIFE:

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RHOGAM

PLT CONCENTRATE / PLTPHERESIS

5 days at RT WITH agitation

GRANNULOCYTE CONCENTRATE

24 Hrs at RT WITHOUT agiitation

10yrs

Frozen RBCs

IRRADIATED BLOOD

Essential for pxns at risk from TA-GVHD

Recipients of plts selected for HLA or plt

Recipients of donor units from blood relatives

CESIUM 137 OR COBALT 60

IRRADIATTED BLD uses :

irradiated at 25-35 Gy dose

28 days

RBC expires ____ after irradiation or at the end of th storage period, whichever comes first

48H / 2DAYS

DEFERRALS:

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ASPIRIN

2wks

DEFERRALS

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Yellow Fever

Mumps

Measle

Typhoid fever

Polio vaccine

Vaccination