gel electrophoresis

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Last updated 6:24 PM on 6/5/23
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9 Terms

1
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rflp process

1. extraction (obtain dna)
2. restriction (cut dna into fragments)
3. separation (gel electrophoresis)
2
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how do you cut dna
restriction enzymes
3
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electrophoresis
a method of separating molecules by placing them in a gel through an electric field
4
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purpose of gel electrophoresis
separate DNA, RNA, and proteins
5
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what charge is dna
negative
6
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how much do the smallest and largest fragments travel
smallest - farthest

largest - least
7
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why add buffer
conducts electricity
8
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fill micropipette

1. depress
2. hold
3. insert
4. release
5. remove
9
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fill gel

1. insert
2. depress
3. hold
4. remove
5. release