gel electrophoresis

rflp process:

1. extraction

• obtain dna

1. restriction

• cut dna into fragments by restriction enzymes

1. separation

• gel electrophoresis

electrophoresis: a method of separating molecules by pacing them in a gel through an electric field

• used to separate DNA, RNA, and proteins

dna is negatively charged

• attracted to positive end of gel

separation is based on size of fragments

• smallest travel furthest
• largest travel least

buffer

add buffer solution because it conducts electricity

micropipette:

• holds exactly 40 microliters
• never hold upside down

fill micropipette:

1. depress
2. hold
3. insert
4. release
5. remove

fill gel

1. insert
2. depress
3. hold
4. remove
5. release