Chapter 9: Visualizing Cells

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14 Terms

1

Light Microscopy

A technique that uses visible light and magnifying lenses to observe and study cells and their structures.

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2

Brightfield Microscopy

The most common type of light microscopy that uses transmitted white light to illuminate the sample, resulting in a dark specimen against a bright background.

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3

Fluorescence Microscopy

A type of microscopy that utilizes fluorescent molecules to visualize specific structures or molecules within cells.

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4

Confocal Microscopy

A specialized form of fluorescence microscopy that provides optical sectioning and improved resolution by eliminating out-of-focus light.

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5

Time-Lapse Microscopy

The capture of images of cells or tissues at regular intervals over a defined period to observe dynamic processes.

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6

Immunofluorescence

A technique that combines the specificity of antibodies with fluorescence microscopy to label specific proteins or molecules.

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7

Live-Cell Imaging

The observation of living cells over time using specialized microscope setups with environmental control.

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8

Resolution

The level of detail that can be observed in a microscope image.

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9

Electron Microscopy

A powerful imaging technique that uses a beam of electrons instead of light to visualize cells and molecules at high resolution.

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10

Transmission Electron Microscopy (TEM)

A type of electron microscopy that involves passing a beam of electrons through an ultrathin sample to generate an image.

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11

Scanning Electron Microscopy (SEM)

A type of electron microscopy that involves scanning a focused beam of electrons across the surface of a sample to generate a 3D image.

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12

Cryo-Electron Microscopy (Cryo-EM)

A technique that allows for imaging samples in their near-native, frozen-hydrated state.

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13

Electron Tomography

A technique used to create 3D reconstructions of cells and cellular structures by capturing a series of images from different tilt angles.

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14

Single-Particle Analysis

A method used in cryo-EM to determine the 3D structures of macromolecules by imaging individual particles and aligning them to reconstruct a structure.

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