Untitled Flashcards Set

Culture media

Solid, liquid, or semi-solids used to support the growth of microorganisms.

Liquid

What state of matter does culture media broth exist in?

Solid, Liquid (broth), Semi-solid

What are the classifications of culture media according to consistency?

1.5-3% of agar

How much solidifying agent is added to solid culture media?

Agar

What is the most commonly used substance as a solidifying agent?

Greater than or equal to 95 °C

What is the melting point of agar?

Less than 50 °C

At what temperature does agar resolidify?

Solid

Which type of culture media is used only to identify one type of specific microorganisms?

Solid

Which type of culture media is used for surface growth of microorganisms to observe colony appearance?

Solid

Which type of culture media is used for pure culture isolations?

Solid

Which type of culture media is used for storage cultures?

Solid

Which type of culture media is used to observe specific biochemical reactions?

Turbid

What is the state of liquid broth when there is growth?

Clear

What is the state of liquid broth when there is no growth?

Liquid/Broth

Which type of culture media can be used to propagate large numbers of microorganisms in fermentation tubes?

Liquid/Broth

Which type of culture media can be used for various biochemical tests?

Less than 1.5% of agar

How much solidifying agent is added to semi-solid culture media?

Semi-solid

Which type of culture media is used for fermentation studies?

Semi-solid

Which type of culture media is used in determining bacterial motility?

Semi-solid

Which type of culture media is used in promoting anaerobic growth?

Semi-solid

Which type of culture media uses a wireloop or wirestick?

Enrichment media

Culture media according to function that contains specific nutrients for the enhancement of growth of a particular pathogen from a mixture of organisms

Nutrient specificity

What concept does enrichment media use to enhance the growth of a specific pathogen?

selective media

Culture media that favors the growth of a particular desired bacteria by inhibiting the growth of undesired bacteria.

enriched medium

Culture media that is enriched usually by adding blood or eggs (albumin).

Culture media that contains nutrients to support the growth of most non-fastidious organisms without giving any particular organism a growth advantage.

What is supportive/nonselective medium?

Culture media that is used to distinguish bacteria from each other on the same agar plate.

What is differential medium?

1. Weigh the powder 2. Dissolve 3. Dispense 4. Sterilize

What are the steps in tubed media preparation?

1. Weigh the powder 2. Dissolve 3. Sterilize 4. Dispense

What are the steps in plated media preparation?

The process of rendering a medium or material free of all forms of life.

What is sterilization?

Sterilization that exposes media to steam at 121°C and 15 lbs of pressure for 15 minutes or longer.

What is autoclaving?

121 °C

What is the temperature of the steam in autoclaving?

15 lbs

What is the pressure used for autoclaving?

A method that uses 260nm Ultra violet radiation to kill most microorganisms, but does not penetrate effectively.

What is UV radiation in sterilization?

260nm

What is the wavelength of the UV radiation in sterilization?

A sterilization method that rapidly penetrates packing materials, including plastic wraps.

What is ethylene oxide?

Crystal violet, gram's iodine, alcohol, safranin red.

What are the stains used in gram staining?

30 seconds

How long is crystal violet used in gram staining?

1 minute

How long is gram's iodine used in gram staining?

5-15 seconds

How long is alcohol used in gram staining?

1 minute

How long is safranin red used in gram staining?

Colorless.

What is the color of gram negative bacteria once alcohol is used?