Untitled Flashcards Set

Solid, liquid, or semi solids usually bought in reagent bottles in powdered form that are used to support the growth of microorganisms

Culture media

In what state of matter does culture media broth exist in

liquid

Classifications of culture media according to consistency

Solid, Liquid(broth), Semi-solid

How much solidifying agent is added to solid culture media

1.5-3% of agar

The most commonly used substance as a solidifying agent

Agar

melting point of agar

Greater than or equal to 95℃

Temperature where agar resolidifies

Less than 50 ℃

Culture media according to consistency that is used only to identify one type of specific microorganisms

Solid

Culture media according to consistency that is used for the surface growth of microorganisms to observe colony appearance

Solid

Culture media according to consistency that is used for pure culture isolations

Solid

Culture media according to consistency used for storage cultures

Solid

Culture media according to consistency used to observe specific biochemical reactions

Solid

What is the state of the liquid broth when there is growth

Turbid

What is the state of the liquid broth when there is no growth

Clear

Culture media according to consistency that can be used to propagate large numbers of microorganisms in fermentation tubes

Liquid/Broth

Culture media according to consistency that can be used for various biochemical tests

Liquid/Broth

How much solidifying agent is added to semi-solid culture media

less than 1.5% of agar

Culture media according to consistency that is used for fermentation studies

Semi-solid

Culture media according to consistency that is used in determining bacterial motility

Semi-solid

Culture media according to consistency used in promoting anaerobic growth

Semi-solid

Culture media according to consistency that uses a wireloop or wirestick

Semi-solid

Culture media according to function that contains specific nutrients for the enhancement of growth of a particular pathogen from a mixture of organisms

Enrichment media

Concept enrichment media uses to enhance the growth of a specific pathogen in a mixture of microorganisms through the nutrients it contains

Nutrient specificity

Culture media according to function that favors the growth of a particular desired bacteria by inhibiting the growth of undesired bacteria

Selective media

Culture media according to function that is enriched usually by adding blood or eggs(albumin)

Enriched medium

Culture media according to function that contains nutrients to support the growth of most non-fastidious organisms without giving any particular organism a growth advantage

Supportive/nonselective medium

Culture media according to function that is used to distinguish bacteria from each other on the same agar plate

Differential medium

Tubed media preparation steps(1-4)

1. Weigh the powder

2. Dissolve

3. Dispense

4. Sterilize

Plated media preparation steps(1-4)

1. Weigh the powder

2. Dissolve

3. Sterilize

4. Dispense

The process of rendering a medium or material free of all forms of life

Sterilization

Sterilization that exposes media to steam at 121C and 15lbs of pressure for 15mins or longer, depending on the nature of the item

Autoclaving

Temperature of the steam in autoclaving

121 C

Pressure used for autoclaving

15 lbs

Sterilization method that uses 260nm Ultra violet radiation, which is lethal to most microorgnaisms but does not penetrate glass, dirt films, water, and other substances effectively

UV radiation

wavelength of the UV radiation in sterilization

260nm

Sterilization method to kill both microorganisms and spores. It is particularly effective as a sterilizing agent because it rapidly penetrates packing materials, even plastic wraps

Ethylene oxide

The stains used in gram staining

Crystal violet, gram's iodine, alcohol, safranin red

length of time crystal violet is used

30 sec

length of time gram's iodine is used

1min

length of time alcohol is used in staining

5-15sec

length of time safranin red is used

1min

Color of gram negative bacteria once alcohol is used

Colorless