Investigate the effect of IAA growth regulator on plant tissue

Step 1

Label 8 bottles A-H

Step 2

Transfer 10ml of stock solution into bottle A. Pour 9ml of water to bottles B-H

Step 3

Drop 1ml of IAA from A to B and stir. Repeat this trasnferring 1ml from B to C, C to D etc until G

Step 4

Remove 1ml from bottle G and dispose of it

Step 5

Place acetate grids in the lid of 8 petri dishes

Step 6

Place cress seeds along the grid lines of each

Step 7

Place filter paper on the seeds in each dish

Step 8

Add a few drops of each IAA solution to each filter paper.

Step 9

Cover filter papers with wool

Step 10

Pour rest of IAA solutions onto each piece of cotton wool

Step 11

Tape the petri dishes shut

Step 12

Stand petri dishes horizontally

Step 13

Leave dishes in an incubator at 20-25 degrees for a few days

Step 14

Use acetate grid to measure shoots and roots and record results

What is this dilution process known as?

Serial dilution

Why are the petri dishes placed horizontally?

Ensures roots grow down and shoots grow up