ADHESIVES AND MOUNTING MEDIA- LABORATORY

ADHESIVES AND MOUNTING MEDIA

essential for methods that require exposure of sections to acids and alkali (ammoniacal silver solutions)

MAYER’S EGG ALBUMIN

Egg whites and Glycerin, Add Thymol crystals for antifungal effect. Convenient, relatively inexpensive

DRIED ALBUMIN

Dried albumin, Sodium Chloride, Dissolve in 100 cc of Distilled water. Add thymol crystals

GELATIN 1%

Gelatin, Distilled Water, Glycerol, Phenol Crystals

GELATIN FORMALDEHYDE

1% Gelatin and 2% Formaldehyde

STARCH PASTE

Powdered Starch, Distilled Water, Hydrochloric Acid, Thymol Crystals

PLASMA

Human plasma from expired blood bag units. Dispensed into sterile tubes of 0.5 ml each.

POLY-L-LYSINE

Aqueous detergent (0.1% solution) diluted in 1:10 ratio with distilled water. For immunohistochemistry

APES

Or 3-Aminopropylthriethoxysilane, useful in cytology, cytospin of proteinaceous or bloody

fluids

MOUNTING MEDIA

prevents the movement of coverslip, protects the stained section from scratches, bleaching or deterioration.

Prevents distortion of microscopic images.

MOUNTING MEDIA

The refractive index of the mountant should be as near as possible to that of the glass which is 1.518

MOUNTING MEDIA

Freely miscible with xylene and toluene

Aqueous and Resin type media

MOUNTING MEDIA- Two types are used:

WATER

Low refractive index, temporary mounting

GLYCERIN

High refractive index (1.46)

GLYCERIN

Consisted of Gelatin, Glycerol, Distilled Water and Phenol Crystals (preservative)

Glycerin Jelly

Standard Mounting Media (used when dehydration and clearing with Xylene cannot be made)

FARRANT’S MEDIUM

Refractive Index of 1.43

FARRANT’S MEDIUM

Consisted of Gum Arabic, Distilled Water, Glycerol and Sodium Merthiolate (Arsenic Trioxide can be used as a substitute)

APATHY’S MEDIUM

Refractive Index of 1.52

APATHY’S MEDIUM

For methylene-blue stained nerve preparations

APATHY’S MEDIUM

Add 50 grams of Potassium Acetate or 10 grams Sodium Chloride to prevent

APATHY’S MEDIUM

Bleeding of metachromatic stains for Amyloid

BRUN’S FLUID

Mounting frozen sections from water

CANADA BALSAM

Refractive Index of 1.524

CANADA BALSAM

Came from Abus balsamea (Canadian Tree)

CANADA BALSAM

For whole mounts and thick sections

DPX

Refractive Index of 1.532

DPX

For small tissues

XAM

Refractive Index of 1.52

XAM

Synthetic mixture in xylene

CLARITE

Refractive Index of 1.544

CLARITE

Preferred over DPX