Test and Stains

Schaeffer & Fulton

Stain used to demonstrate spores

Hanging drop

Presumptive test for Listeria

Anthony’s, Muir, Hiss, Tyler

“Si Anthony at MUIRis, nag kHISS sa Tyler matapos malagyan ng CAPSULE ang beer”

Capsular stains

Grays, Fisher and Conn, Leifson

“Gray Fisher was a CONN man who LEIF his SON”

Flagellar stain

Schaeffer and Fulton

Spore stain

Wayson and Methylene blue

Stain for polar bodies

CNA

Selective for GPC esp from wound and stool culture

Colistin and Nalidixic

Inhibitory component for G- in CNA

MSA

Selective-differential for S. aureus

phenol red

pH indicator of MSA

7.5% salt

Inhibitor of MSA

C. diptheriae

CTBA is used for the isolation of ________and produced gray-black colonies

Tindsdale

C. diphtheriae is an isolation medium for ______ and produces black colonies with brown halo

Loeffler’s medium

Enrichment for C. diphtheriae, promotes development of metachromatic granules

PLET ( Polymyxin Lysozyme EDTA Thallous acetate)

B. anthracis is an isolation medium of

drive off O2

purpose of boiling Thio

Facultative anaerobes

Diffuse, even growth all throughout the media

Vancomycin

Composition of Thayer Martin that inhibits gram +

Colistin

Composition of Thayer Martin that inhibits gram -

Nystatin

Composition of Thayer Martin that inhibits fungi

Trimetophrim

Modified Thayer Martin composition that prevents swarming of organism

Anisomcyin

Component of Martin Lewis that prevents fungi

Thayer Martin

Inhibitory agents: VCN

Modified Thayer Martin

Inhibitory agents: VCN-T

Lowenstein-Jensen Media

Most common media for MTB

M. tuberculosis

Petragnani is recommended for non-sterile culture of _______

Eosin Y and Methylene blue

Inhibitor and Indicator for EMB

Crystal violet and Bile Salts

Inhibitor of MAC

Neutral Red

Indicator MAC

Bile salts

Inhibitor of SSA, XLD and HEA

phenol red

Indicator of XLD

bromthymol blue

Indicator of HEA

BCDA (Buffered Charcoal Yeast Extract Agar with Antibiotics)

For growing Francisella Tuarensis

Bordet-Gengou Media

Isolation of Bordetella pertussis

BCYE (Buffered Charcoal Yeast Extract)

Enrichment and Selective Media for Legionella spp

Casein

Used for separating Nocardia and Streptomyces. Made from skimmed milk

Tomato juice agar

Media used for Lactobacillus acidophilus

SDA

Used for fungus culture

Regan Lowe

Enrichment and selective media media for Bordetella pertussis

Deoxycholate Agar

Used for gram negative enteric bacilli differentiating lactose fermenters from nlf

Corn Meal Agar with 1% Tween 80

Used to stimulate production of Chlamydospores of Candida albicans

Catalase Test

To differentiate genus Staphylococcus and Micrococcus from Streptococcus

Coagulase Test

Definitive test for S. aureus

Slide Coagulase

To detect bound coagulase or clumping factor

Tube coagulase

test used to detect free coagulase in a liquid medium.

Modified Oxidase Test

Presumptive identification of Micrococcus. Rapid method to differentiate Micrococci (+) from Staphylococci (-)

tetramethyl-p-phenylenediamine dihydrochloride in DMSO

Modified Oxidase Test uses ______ as reagent

S. aureus

(+) control in Catalase and Coagulase Test

S. pyogenes

(-) Control in CatalasTest

S. epidermidis

(-) Control in Coagulase test

mecA gene

Gold standard involves detection of ____

Rose to Mauve Colony Color

MRSA result in Chrom-Agar