Techniques for investigating the limiting factors on rate of photosynthesis

limiting factor

any resource that could potentially limit continuation of a reaction

conditions photosynthesis is dependent on

• temperature

• light intensity

• CO₂ concentration

temperature and the rate of photosynthesis

light intensity and the rate of photosynthesis

CO₂ temperature and the rate of photosynthesis

method 1 (measuring rate of photosynthesis)

measuring CO₂ uptake

how is mthod 1 used to measure rate

increased CO₂ uptake by plant will lower the concentration, increasing alkalinity which is then measured by a pH probe

method 2:

measuring O₂ production

how does method 2 measure the rate

oxygen from the plant can be trapped and measured with a gas syringe

method 2 alternative process

oxygen production can be measure by the time taken for submerged leaf discs to surface