Practical 3: Fat Content

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5 Terms

1
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Why is the Soxhlet method unsuitable for moist samples?

Moisture interferes with solvent efficiency in lipid extraction.

2
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How does the Mojonnier method differ from Soxhlet in fat extraction?

It uses acid hydrolysis and diethyl ether for moist samples like meat.

3
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What is the role of 4N HCl in the Mojonnier method?

It breaks down protein and cell structures to release fat.

4
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Why must samples be predried before Soxhlet extraction?

To prevent interference of moisture with lipid solubility in hexane.

5
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How is % fat content calculated?

% Fat = [(Flask + fat) − (Flask)] / Sample weight × 100