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Flashcards on recalled topics from the March 2023 MTLE exams, covering Clinical Microscopy, Histopathology, MT Laws, Hematology, Blood Banking, Immunology, Serology, Microbiology, Parasitology, and Clinical Chemistry.
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Total magnification for grading casts
10x
Pregnancy (POCT) test kit uses what principle:
Immunochromatography
What does PASS stand for when operating fire extinguishers?
Pull pin, Aim at the base of the fire, Squeeze handles, Sweep nozzle side to side
What does RACE stand for when discovering a fire?
Rescue, Alarm, Contain, Extinguish/Evacuate
Reference Values for Semen Analysis: Volume
2 to 5 mL
Reference Values for Semen Analysis: Sperm concentration
20 million/mL
Reference Values for Semen Analysis: Sperm count
40 million/ejaculate
Reference Values for Semen Analysis: Motility
50% within 1 h
Reference Values for Semen Analysis: Round cells
<1.0 million/mL
Liquefaction (Semen Analysis)
A fresh semen specimen is clotted and should liquefy within 30 to 60 minutes after collection
Sperm Morphology
Sperm morphology is evaluated with respect to the structure of the head, neckpiece, midpiece, and tail.
Measurement of a -glucosidase is performed to detect a disorder of the:
Epididymis
Kohler illumination
Type of microscopic illumination in which a lamp condenser focuses the image of the light source onto the front focal plane of the substage condenser.
Most common contamination in urine from female patients if it is NOT collected using the midstream clean-catch technique:
squamous epithelial cells
Clue Cells
Clue cells are indicative of vaginal infection by the bacterium Gardnerella vaginalis.
Cylindroids
Cylindroids have the same significance as casts.
Cast disintegration is based on?
Hypotonic and alkaline urine promotes the disintegration of casts in the urine sediment.
Protein range (mg/dL) for grade of 2+
100–200
Urine Clarity
Freshly voided normal urine is usually clear
Classic test for differentiating between urobilinogen, porphobilinogen, and Ehrlich-reactive compounds
Watson Schwartz test
First morning urine is not required in:
urine culture
Copper sulfate tests the _ ability of glucose:
reducing
Copper Reduction Test (Clinitest)
Measurement of glucose by the copper reduction method was one of the earliest chemical tests performed on urine. The test relies on the ability of glucose and other substances to reduce copper sulfate to cuprous oxide in the presence of alkali and heat.
Staghorn calculi:
hard large branching
Antiglomerular basement membrane antibody is seen with:
Goodpasture syndrome
The only protein produced by the kidney is:
Uromodulin
To distinguish yeast cells from RBC:
Yeast cells usually exhibit budding.
Nuclear detail can be enhanced by:
Acetic acid
NORMAL odor of urine
ammoniacal
A significant rise in the OD of amniotic fluid at 450 nm indicates the presence of which analyte?
Bilirubin
Amniotic fluid is:
Amniotic fluid is a product of fetal metabolism.
Baby powder (lycopodium) crystal looks like ____ under microscope
*Talcum powder crystals are rounded with a small cross in their center.
What should be done in a refrigerated specimen with presence of pink precipitates before testing?
*Warm
Bloody CSF indicative of intracranial hemorrhage:
Even distribution of blood in all tubes
CSF collection:
3rd, 4th, 5th Lumbar
Non-invasive procedure for gastric analysis:
*Urea breath test
Positive color of Ictotest:
blue-to-purple
Fluid with low protein
Transudate
Fluid with high protein
Exudate
Which of the following parameters best identifies a fluid as a transudate or an exudate?
Total protein ratio and lactate dehydrogenase ratio
Tubercular peritonitis ~lab tests
Decreased glucose,
Acid-fast stain,
Adenosine deaminase
A web-like pellicle in a refrigerated CSF specimen indicates:
Tubercular meningitis
A CSF Total count is diluted with:
Acetic acid
WBC count
Which is considered abnormal when present in feces?
RBCs
Storage for cell blocks
Many years
Which can perform red cell antibody screening?
Blood bank
Fixatives
The aldehydes (formaldehyde, glutaraldehyde) and oxidizing agents (osmium tetroxide, potassium permanganate) act by cross-linking proteins.
Fixatives for electron microscopy:
Glutaraldehyde
Osmium tetroxide
When to perform decalcification?
AFTER fixation
BEFORE impregnation
Defining standards of performance:
Directing
Planning
Organizing
Controlling
Not a fibrocartilage:
*ligaments
Not considered as a laboratory record:
*Laboratory worksheet
Antiseptic for blood alcohol analysis should be:
non-alcohol based
What should be first secured by the owner from the DOH to operate a lab?
*Permit to construct and design
Who can perform HIV testing?
Medtech with HIV proficiency cert
Minimum requirement of lab personnel for screening drug laboratory:
analyst, head pathologist, authorized specimen collector
Who regulates the reagents being used in the laboratory?
FDA
Who cannot practice medical technology in the Philippines?
No medical technologist shall practice or offer to practice medical technology as defined in RA 5527 without having previously obtained a valid certificate of registration from the Board
Why PCR testing is done:
Amplify small amounts of DNA
Rinse hands
Rinse hands in a downward position to prevent recontamination of hands and wrists
Fibrinogen disorder
Dysfibrinogenemia
Morphologic changes occur during normal blood cell maturation
Decrease in cell size, Decrease in nuclear diameter, loss of nucleoli, condensation of nuclear chromatin, and decreased basophilia in cytoplasm
In what cell type are Niemann-Pick cells?
Macrophage
MOST affected by excessive anticoagulant
*HCT
What light source is used in vein illumination devices (like AccuVein)?
near infrared
From the following, identify a specific component of the adaptive immune system that is formed in response to antigenic stimulation
Immunoglobulin
Which cluster of differentiation (CD) marker appears during the first stage of T-cell development and remains present as an identifying marker for T cells?
CD2
All of the following are immunologic functions of complement except
Induction of an antiviral state
Which serum antibody response usually characterizes the primary (early) stage of syphilis?
Antibodies against syphilis are undetectable
Which tests are considered screening tests for HIV?
ELISA, 4th generation, and rapid antibody tests
Interpret the following results for HIV infection: ELISA: positive; repeat ELISA: negative; Western blot: no bands
Negative for HIV
Which CD4:CD8 ratio is most likely in a patient with AIDS?
1:2
What should be done if all forward and reverse ABO results as well as the autocontrol are positive?
Wash the cells with warm saline, autoadsorb the serum at 4oC
The k (Cellano) antigen is a high-frequency antigen and is found on most red cells. How often would one expect to find the corresponding antibody?
Rarely
Which of the following is note requirement for the electronic crossmatch?
There are concordant results of at least one determination of the recipient’s ABO type on file
What ABO types may donate to any other ABO type?
O-
A major crossmatch and screening cells are 2+ at IS, 1+ at 37°C, and negative at the IAT phase. Identify the most likely problem.
Cold alloantibody
All of the following are reasons for performing an adsorption, except:
Confirmation of weak antigens on red cells
What should be done when a woman who is 24 weeks pregnant has a positive antibody screen?
Perform an antibody identification panel; titer if necessary
When may an immediate spin crossmatch be performed?
4 year history. no fever, what diagnosis?
ANA tests are performed to help diagnose which condition:
SLE
What of recombinant vaccinations carries on deferral pertussis:
Rubberella
In chronic active hepatitis, high titers of which of the following antibodies are seen?
*Anti-smooth muscle
Anti-glomerular basement membrane antibody is MOST often associated with this condition:
Goodpasture disease
Immunoglobulin that is most efficient at crossing the placenta:
IgG
Which test is used to evaluate the cellular immune system in a patient?
Skin test for commonly encountered antigens
If the number on the CD8 surface in the patient in high, what is the next checkup?
Immunosuppressants
Symptoms of dyspnea, cough, hypoxemia, and pulmonary edema within 6 hours of transfusion:
TRALI
Which of the following white blood cells is capable of further differentiation in the tissues? Stevens
Monocyte
Which of the following statements accurately describes competitive binding assays? Stevens
the concentration of patient analyte is inversely proportional to bound radioactive label.
Plastic bag overwraps are recommended when thawing units of FFP in 37oC water baths because they prevent:
All COCCI, what is Gram positive
A 10% red cell suspension in saline is used in a compatibility test. Which of the following would most likely occur?
False-negative due to prozone
Nontremono
The following is a treponemal test:
T cells cannot be, What cellular function
AHA, HEP/AIDS/DMS
When is a patient be mass transfusion, how match, and what procedure?
Blood Banking: Calculations for collection
Which of the best of the following test in the lab:
The most common test milk quality:
Thyroxine the Largest amount
The T4 is stored when:
What about test of blood bank or immuno can came up in last exam:
Harr and BOC book - Questions March MTLE:
Samples (2) for detection of microsporidia:
Fresh, Formalin-fixed
Actinomyces
Gram-positive bacilli