evobio: lec 4-5 (fruit flies proteins)

48

The number of orthologues of known D. melanogaster sperm proteins identified is:

3096

The total number of non-redundant proteins identified from reproductive tissues is:

1453

The number of proteins identified in males is:

762

The number of proteins identified in mated females is:

437

The number of proteins identified in unmated females is:

141

The number of proteins present in males and mated females but absent in unmated females is:

1

Among the 141 transferred proteins, the number that is an orthologue of a known Drosophila melanogaster seminal fluid protein is:

FBgn0023529

The gene ID of the identified Drosophila melanogaster seminal fluid protein orthologue is:

sperm protein

The identified seminal fluid protein orthologue (FBgn0023529) is also classified as a:

569

The number of proteins identified in male accessory glands (MAG) is:

100

The number of male accessory gland proteins transferred to females during mating is:

Nucleotide binding

A functional cluster involving binding to nucleotides is:

Hippo signaling pathway

A functional cluster related to a signaling pathway is:

Methylation

A functional cluster involving addition of methyl groups is

ATPase activity

A functional cluster associated with enzyme activity involving ATP hydrolysis is

scribble

The protein that has an orthologue in Drosophila involved in memory, behavior, and synapse structure is:

shibire

The protein associated with microtubules and sperm tail structure is:

Smi97B

The allele of Scribble that causes olfactory deficit in fruit flies when mutated is:

Ago2

The proteins transferred that are immune-related include Ras85D, Dicer2, Rab6, Fat, Psdin, Rhol, aPKC, and:

Ras85D

The protein involved in ERK signaling and stress response is:

Dicer2

The protein associated with antiviral defense and modulation of immune signaling is

Rab6

The protein involved in antifungal defense and vesicle trafficking is:

Psdin

The protein that is lysosomal in function and degrades bacteria while inducing defensins is:

Fat, RhoL, and aPKC

The proteins associated with hemocyte release are:

Ras85D

The lifespan-related proteins transferred include Lam, Widerborst, and:

Lam

The protein that shortens lifespan when overexpressed is:

Widerborst

The protein that increases lifespan is:

Ras85D

The protein that regulates lifespan is:

Oogenesis

The process of egg development is called:

Spermatogenesis

The process of sperm development is called:

601

The total number of proteins identified in testes is:

69

The number of proteins transferred to females from testes is:

283

The total number of proteins identified in the male ejaculatory bulb is:

20

The number of proteins inferred to be transferred to females during mating from the ejaculatory bulb is:

2

The number of individual proteins highlighted from the ejaculatory bulb is:

Porin

The protein associated with sperm individualization and mitochondrial morphology is:

Ejaculatory bulb

The organ from which Porin is transferred is:

11

The number of proteins identified in male reproductive organs that are orthologous to Drosophila melanogaster oogenesis proteins is:

Dhc64C

The protein associated with oocyte differentiation is:

Ras

The protein associated with follicular epithelium patterning and eggshell shape is:

Msp-300

The protein involved in egg chamber development and cytoplasmic dumping is:

PI4KIIIα

The protein associated with border cell migration and nurse cell dumping is:

l(2)gl

The protein associated with cortex formation and oocyte polarization is:

Rab6

The protein associated with organization, growth, and polarization is:

scribble

The protein associated with follicle cells is

RhoL

The protein associated with egg chambers is:

SesB

The protein involved in autophagy via mitochondrial stress regulation is:

6

The number of proteins identified that are orthologous to Drosophila spermatogenesis proteins is:

Porin

The protein associated with spermatid individualization is:

Lam

The protein associated with testes size is:

Bel

The protein associated with spermatid nuclei size and flagella is:

Nap1

The protein associated with sperm chromatin condensation is:

aPKC

The protein associated with polarization of sperm cells is:

Bactrocera oleae

In _____, manipulation of two reproductive genes led to a significant decrease in oviposition rate, showing that genetic interventions can reduce egg production after mating.

Aedes aegypti

In ____, knockout of β2‑tubulin eliminated male fertility, showing genetic manipulation can induce sterility.

Drosophila

• In ___, Porin and Bel mutants are sterile, confirming their importance in sperm development.

Shibire

The protein associated with sperm individualization is: