white light microscopy and staining
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Last updated 9:36 PM on 10/21/24
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8 Terms
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different types of light microscopy
brightfield
darkfield
phase contrast
differential interference contrast
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feature of phase contrast
white halo around sample
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feature of DIC
3D pseudo relief, light and dark sides
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what do hematoxylins stain
they stain negatively charged molecules (DNA,RNA)
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eosins stain
they stain positively charged molecules (cytoplasm, membrane, ECM)
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colour of hematoxylins
purple
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colour of eosins
pink
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immunohistochemistry (IHC)
enzyme conjugated to antibody targets antigen
substrate is cleaved = leads to stained precipitate
helps us to detect specific proteins in a sample
Note 
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