white light microscopy and staining

0.0(0)
studied byStudied by 0 people
learnLearn
examPractice Test
spaced repetitionSpaced Repetition
heart puzzleMatch
flashcardsFlashcards
Card Sorting

1/7

encourage image

There's no tags or description

Looks like no tags are added yet.

Study Analytics
Name
Mastery
Learn
Test
Matching
Spaced

No study sessions yet.

8 Terms

1
New cards

different types of light microscopy

  • brightfield

  • darkfield

  • phase contrast

  • differential interference contrast

2
New cards

feature of phase contrast

white halo around sample

<p>white halo around sample</p>
3
New cards

feature of DIC

3D pseudo relief, light and dark sides

<p>3D pseudo relief, light and dark sides</p>
4
New cards

what do hematoxylins stain

they stain negatively charged molecules (DNA,RNA)

5
New cards

eosins stain

they stain positively charged molecules (cytoplasm, membrane, ECM)

6
New cards

colour of hematoxylins

purple

7
New cards

colour of eosins

pink

8
New cards

immunohistochemistry (IHC)

  • enzyme conjugated to antibody targets antigen

  • substrate is cleaved = leads to stained precipitate

  • helps us to detect specific proteins in a sample

Explore top notes

Explore top flashcards