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maximum wavelength absorbance
The ________ (lambdamax) in a sample is used to calculate the protein concentration.
amino acids
A chain of bonded ________ is called a polypeptide.
Sodium Dodecyl Sulfate
________ (SDS detergent) binds to the proteins to make them uniformly negatively charged.
Proteins
________ are complex macromolecules that are made up of long chains of amino acids.
Enzymes
________ are biological catalysts that speed up chemical reactions in living organisms.
Mass spectrometers
________ can be used to identify unknown compounds, to quantify known compounds, and to determine structure /chemical properties of molecules.
peptide bonds
In order to form ________, the carboxyl group of one amino acid reacts with the amino group of another.
polypeptide chain
The ________ folds due to hydrogen bonds.
absorption of light
Measures the protein concentration in a solution using the transmittance of light and the ________ in a protein sample.
Protein samples
________ are ran on a PAGE gel then transferred to a blotting membrane.
disulfide bonds
Interactions in the R groups of the helixes and sheets cause ________.
Proteins
________ are chains of amino acids joined together by peptide bonds.
Enzymes
________ are highly specific, and can only work with certain substrates.
Bonds in the secondary structure
Hydrogen bonds
Bonds in the tertiary structure
Disulfide bonds
Reaction where the carboxyl bonds to the amino
Dehydration synthesis or condensation
Quaternary Structure
Multiple polypeptide chains
Bradford Assay
Coomassie dye reacts with proteins to produce color → the more color the more protein
How does temperature affect enzymes
Breaks H bonds and causes denaturing
How does pH affect enzymes
Affects charges on side chains and denatures enzymes
How can we extract and purify proteins
Chromatography!!!!!
X-Ray crystallography
