coochie and ear cytology (copy)

Define estrous cycle

Puberty

Stages of estrous

Proestrus

Estrus

Metestrus/ Diestrus

Anestrus

Proestrus symptoms / duration

Swollen vulva

Bloody vaginal discharge

Becomes a tease

Urine marking

duration of proestrous

Average 7-10 days (9 days)

Range 4-25 days

Estrus symptoms / duration

Vulva is less swollen

Less bloody discharge

Straw coloured discharge

Urine marking

On the hunt for a lover, and the guys be noticing and wanting to engage. Bow chick a wow wow time

duration of estrus

average- 3-10 days

range 2-15 days

Metestrus / diestrus symptoms / duration

Normal cooch

Mucous to dark brown color initially

May have morning sickness for a few days if prego

Can undergo false pregnancy

duration of metestrus/ diestrus

70-80 days

Anestrus symptoms / duration

Normal cooch

Period of inactivity between end of diestrus and the onset of the next Proestrus

Anestrus duration

4-5 months

How do you determine ovulation

Vag cytology

LH determination

Serum progesterone determination

When would you get increased estrogen results on a vag cytology

Bloody vaginal fluid (diapedesis)

Epithelial cell proliferation and differentiation (thickening of epithelium or keratinization of cells)

what species are vaginal cytologies most commonly used to stage the estrous cycle?

dogs

How to take a vag smear

Moisten cotton swab

Use speculum

Touch vaginal wall with swab, do not scrape

Avoid collecting cells from vestibule or clitoral fossa

Roll swab on slide when removed

What do you stain vaginal cytology with?

sedi stain

Diff Quik (most common)

Classifications of vaginal epithelial cells

Cornified/ keratinized cells: dead/ dying cells

Non-cornified cells : living cells

Cornified cells

superficial cells

anuclear cells

Non- cornified cells

intermediate cells

parabasal cells

basal cells (rare)

Describe as epithelial cells become Cornified (keratinized) cells

size increases

Nucleus changes from oval to faded, broken, pyknotic as the cell dies it then disappears

Cytoplasm changes from dark blue to light blue purple, then dark blue again as the cell dies

2 other variety of parabasal cells

Foam cells

Metestral cells

On cytology on Proestrus what would you see

High RBC

Increased Cornified cells

On cytology on estrus what would you see

Few or no RBCs/wbcs

Nearly 100% Cornified cells

On cytology on Metestrus / diestrus what would you see

High wbc

Metestrum / foam cells

30% Cornified cells

On cytology on Anestrus what would you see

Few wbcs

30% Cornified cells

What dis

anuclear cell

What dis

superficial

What dis?

parabasal cell

whats this

intermediate cells

What cell dis

Foam cell

What cell dis

Metestral cells

What estrous stage is this

Proestrus

What estrous stage is this

Estrus

What estrous stage is this

Metestrus/ diestrus

What estrous stage is this

Anestrus

What to look for on 10x for ear cytology

Epithelial cells

Mites/ova

Tumor cells

Fungal hyphae

What to look for on 40 x and 100x for ear cytology

Organisms- rods, cocci, yeast

WBC (= infection / inflammation)

RBC (=hemorrhage with trauma, infection, tumor)

What dis

yeast

What dis

Keratinocyte with melanin granules

Who’s all at this party

rods

Cocci

Neutrophils

Who’s at this party

Melanin granules

Nuclear streaming

Cocci

Neutrophils