Photosynthesis Flashcards

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All information on photosynthesis!

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How do living organism recieve energy?
From the sun
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How to photosynthetic organisms capture energy?
plants, algae, bacteria, protists: capture light energy and use it to convert carbon dioxide into organic carbon sugars
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What is not required for photosynthesis?
oxygen
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Where does the light energy from the sun go?
it becomes stored energy in molecules of simple sugars
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What is the equation of photosynthesis?
6CO2 + 6H2O + Light energy → \[CH2O\]n +6O2
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How does water enter plants?
through the roots
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How is water tranposted to the leaves?
through the veins
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How does carbon dioxide enter?
through stomata openings in leaves
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After CO2 and water diffuse into cells, where do they go?
into the chloroplasts
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Where does photosynthesis take place?
in the chloroplast
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What is a thylakoid?
A membrane system within the chloroplast which forms interconnected disks that look like flattened sacs
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What are a stack of thylakoid called?
grana
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Why are thylakoids central to photosynthesis?
the molecules the absorb the solar energy are embedded in their membranes
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What fluid surrounds the grana in the chloroplasts?
stroma
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What is the role of the enzymes in the stroma?
to catalyze the conversion of CO2 and water into carbohydrates
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What two sets of reactions make up photosynthesis?

1. Light-dependent reactions
2. Light-independent reactions
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What occurs in the light-dependent reactions?
light energy is trapped and used to generate two high-energy compounds: ATP and NADPH
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What occurs in the light-independent reactions?
the energy of ATP and the reducing power of NADPH are used to make high-energy organic molecules (sugars)
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What is a light-dependent reaction?
* involves a series of reactions that are energized by light
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Where do the light-dependent reactions take place?
in the thylakoid membranes of the chloroplasts
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After the ligh-dependent reaction, where does the energy go?
the energy absorbed by the thylakoid is transferred to carbohydrate molecules
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What are the two main functions of light-dependent reactions?

1. the capturing of light energy
2. using the light energy to make ATP and reduce NADP+
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Where do the light-independent reactions occur?
in the stroma
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What is carbon fixation?
the incorporation of the carbon of CO2 into organic compounds, like glucose that can be used by the plant
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What do light-dependent reactions require?
ATP and NADPH (from light reactions)
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What is the Calvin cycle?
where carbon fixation occurs during a sequence of enzyme catalyzed reactions
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What is a photon?
light behaves as if it were composed of “units” or “packets” of energy that travels in waves
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How are photons characterized?
by a wavelength that is inversely porportional to their energy
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Photons with short wavelengths…
have high energy
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Photons with long wavelengths…
have low energy
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What does the wavelnegth of light determine?
colour
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What is the wavelength for red light?
700 nm
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What is the wavelength of bue light?
470 nm
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What is pigment?
a compound that absorbs certain wavelengths of visible light
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What is a photosynthetic pigment?
a compound that traps light energy and passes it on to other compounds
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What happends to pigment when sunlight is available?
pigments embedded in the thylakoid membranes absorb light energy, initiating light-dependent reactions
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What is the main type of photosynthetic pigment in plants?
Chorophyll
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What are the two types of chlorophyll which chloroplasts contain?

1. Chlorophyll a
2. Chlorophyll b
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What light does chlorophyll a absorb?
blue-green, and red light
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Which type of light is reflected by chlorophyll a?
green light
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What are accessory pigments?
pigments that absorb slightly different wavelengths of light. These pigments can pass on their energy to chlorophyll a
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What type of light does chlorophyll b absorb?
blue-green light
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What type of light does chlorophyll b reflect?
yellow-green light
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What type of light do carotenoids absorb?
blue-green light
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What type of light do carotenoids reflect?
yellow-orange light
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Do pigments absorb light independently?
NO
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What is a photosystem?
Light is absorbed by chlorophyll or accessory pigment molecules that are associated with these proteins in clusters
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Where are photosystems located?
embedded in thylakoid membranes
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What does a photosystem consist of?
antenna complex, and reaction center
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What is the antenna complex?
a web of chlorophyll and accesory pigment molecules embedded in the thylakoid membrane
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What is the purpose of the antenna complex?
is absorbs a photon and transfers the energy from pigment to pigment until it reaches chlorophyll a
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What is the reaction center?
the chlorophyll a molecule
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What happends when the chlorophyll a absorbs energy?
an electron becomes excited and jumped to a higher energy level
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Where does the excited electron go?
is transferred to the primary electron acceptor
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What type of reactions occur within photosystems?
redox reactions
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What is being oxidized in photosystem redox reactions?
chlorophyll
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What is being reduced in photosystem redox reactions?
the primary electron acceptor
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What are the two types of photosystems in the thylakoid membrane?

1. Photosystem I
2. Photosystem II
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What light wavelength can be absorbed in Photosystem I?
* contains chlorophyll a (in reaction center)
* aborbs 700 nm (red light)
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What light wavelength can be absorbed in Photosystem II?
* contains chlorophyll a (in reaction center)
* absorbs 680 nm (red light)
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What are the roles of the photosystems?
they work together as part of the light-dependent reactions
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What is the goal of light-dependent reactions?
to transfer energy of light to ATP and NADPH
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What is photoexcitation?
the absorbance of a photon by an electron of chlorophyll in photosystem II

* jumping into a higher energy level
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What is the ground state of an electron?
before a photon of light strikes a chlorophyll molecule, the chlorophyll electrons are in the lowest possible energy level
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What happeneds when a photon is aborbed by a chlorophyll molecule?
the electron gains energy and jumps to a higher energy level
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When energy is given to the electron, what happeneds?
the electron can break free from an atom of the chlorophyll molecule
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What does chlorophyll do during photoexcitation?
donate an electron
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How does this lost electron get replaced in the chlorophyll?
a molecule of water is split
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What does the splitting of water do?
* releases a pair electrons
* can replace two donated electrons


* results in formation of oxygen and H+ ions in the thylakoid space
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After replacing the electron, what happends?
chlorophyll is able to respond to another photon
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What is a bydroduct made by the splitting of water?
* oxygen
* this makes its way into the surrounding environment
* H+ ions
* play a role in proton gradient
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What is the electron transport chain (ETC)?
When photosystem II transfers the free electron over to a series of proteins inside the thylakoid membrane
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As electrons pass along these proteins, what does the energy from the electrons fuel?
the b6-f complex (membrane pump)

* actively moves H+ ions against their concentration gradient from:

STROMA →THYLAKOID SPACE
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What is created due to the pumping of H+ into thylakoid space?
electrochemical gradient
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What occurs as electrons move to photosystem I?
the electron from the ETC arrives and is regenerized by another photon captured by chlorophyll
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What does the energy from photosystem I drive?
the energy from this electron drives the formation of NADPH (from NADP+) and a H+

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What enzyme is used to turn NADP+ to NADPH during ETC?
NADP reductase
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Where is solar energy stored?
in the electron carriers

* later used to make sugar molecules
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Is the ETC cyclic or noncyclic?
noncyclic because an electron is lost by a reaction center chlorophyll within the photosystem

* does not return back to the system
* flow of electrons is unidirectional
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How many times must the ETC occur?
* NADP+ requires 2 electrons to reduce to NADPH
* entire process happends TWICE per NADP+ reduced
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What is photophosphorylation?
the use of proteins of light to drive the phosphorylation of ADP to produce ATP via chemiosmosis
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What is cyclic photophosphorylation?
* excited electrons only use photosystem I
* Excited electrons leave photosystem I and are passed to an electron acceptor
* They are then passed to the b6-f complex and back to photosystem I
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What does the cyclic pathway generate?
a proton gradient for chemiosmosis ATP synthe
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What is NOT produced in cyclic pathway?
NADPH nor oxygen
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Which pathway is the plant going to use, cyclic or noncyclic?
the activities of these two photophosphorylation pathways vary depending on the amount of ATP and NADPH required in the stroma
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What is chemiosmosis?
* the net movement of protons through ATP synthase to drive the phosphorylation of ADP to ATP
* the protons that accumulate in the thylakoid space contribute to an electrochemical gradent that drives the process
* light energy is required to create the proton gradient so the process is called: photophosphorylation
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What is produced in the light-dependent reactions?
NADPH and ATP
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What do all chloroplast plants contain?
enzymes in the stroma that, with the energy supplied by ATP and NADPH, convert CO2 to carbohydrates
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What did light-independent reactions used to be called?
“dark” reactions
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Why is “dark” reactions an inaccurate name?
these reactions can take place in the presence or absence of light
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What is the purpose of the light-independent reactions?
Carbon fixation
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What are the 3 stages of the Calvin cycle?

1. Carbon Fixation
2. Reduction Reactions
3. Regeneration of RuBP
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What is the key to Carbon fixation?
the chemical bonding of the carbon atom in CO2 to a pre-existing molecule in the stroma (RuBP)
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How many molecules of CO2 are added to how many molecules of RuBP?

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6 molecules of CO2 + 6 molecules of RuBP
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What molecule is used to CO2 to RuBP?
Rubisco
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How many carbon atoms are in RuBP?

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5 carbon sugar
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What is formed when 6 CO2 molecules combine with 6 RuBP molecules?

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6 unstable six-carbon intermediates are formed
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What happends after the intermediates are formed?

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they are instantly split, and their molecules are reorganized to form 12 molecules of PGA

(3-phosphoglycerate)
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How many carbons are in PGA?

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3 carbons each
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What is the Calvin cycle also known as?

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C3 photosynthesis because the first compound produced (PGA)

* Plants that use this method of photosynthesis are C3 plants