Adaptive Immunity Immunoglobulins (B Cells)

What is the Fab region on immunoglobulins?

Variable region

What is the Fc region on immunoglobulins?

Constant region

What results from papain cleavage of immunoglobulins?

2 Fab

1 Fc

What results from pepsin cleavage of immunoglobulins?

F(ab)2

Which immunoglobulin cleavage is just as effective as neutralizing IgG?

Pepsin Cleavage

What refers to the strength of a single antigen binding to a single epitope?

Affinity

What refers to the strength of a multivalent antigen binding to a multiple epitopes?

Avidity

What structure on the antibody is flexible and opens for high avidity?

Hinge

For avidity, are the epitopes different or identical?

Identical

What is the structure on immunoglobulins that bind to the antigen?

CDR (Hypervariable)

Which CDR codes for the variable region?

CDR1 and CDR2

Which CDR codes for the VDJ junction?

CDR3

Which domain on the immunoglobulin is where diversity can be found?

Variable domain

Which domain on the immunoglobulin is responsible for the effector functions?

Constant domain

What are the types of epitopes?

1. Conformational

2. Linear

Which type of epitope loses binding when denatured?

Conformational

Which type of epitope does not lose binding when denatured?

Linear

List the immunoglobulins in the order on a chromosome?

IgM

IgD

IgG

IgE

IgA

Which immunoglobulin augments phagocytosis?

IgG

Which immunoglobulins are involved in neutralization?

IgG, IgM, IgA

Is neutralization Fab or Fc dependent?

Fab dependent

Which immunoglobulins are involved in opsonization?

IgG1, IgG3

Is opsonization Fab or Fc dependent?

Fc dependent

Which immunoglobulins are involved in complement activation?

IgM, IgG1, IgG3

Which immunoglobulins are involved in mucosal transport?

IgM, IgA

Which immunoglobulins are involved in placental transport?

IgG1, IgG2, IgG3

Which immunoglobulins are involved in J-chain?

IgM, IgA

What which immunoglobulin can be dimeric?

IgA

What which immunoglobulin can be pentameric?

IgM

Which immunoglobulin has the highest avidity?

IgM

How does IgA moved in mucosal transport?

IgA binds to Poly-Ig receptor with J-Chain

What does the IgA & Poly-Ig complex cross during mucosal transport?

Epithelium

What does the IgA & Poly-Ig complex enter during mucosal transport?

Lumen

Is immunoglobulin secreted?

Yes

What do immunoglobulins bind to?

Soluble antigens

What do immunoglobulins recognize?

Conformational determinants

What are the B Cell Markers?

CD19 and CD21