SEROIM SEROLOGICAL PIPETTES

Quantitative transfer of reagents
Serial dilutions of specimen

Uses of Serological Pipettes (2)

Frosted ring
Calibration extends to the tip
To Deliver (T.D.)
Color-coded bands

Characteristics of Serological Pipettes (4)

10ml in 1/10

1ml per graduation

10 ml in 1/10
5 ml in 1/10
2ml in 1/10
2 ml in 1/100
1 ml in 1/10
1 ml in 1/100

Most commonly used numerals (6)

Chemically clean

All serological pipettes must be:

Immersed in or rinsed with cold tap water
Soak and wash in hot tap water with lab detergent
5-7 rinses with tap water
3-4 rinses with distilled water
Air dried or dry in hot-air oven

Steps to chemically clean pipettes: (6)

Pipettes should be inspected for broken or chipped ends and any visible dirt or stain

Only the upper third of the pipette should be touched by fingers

Clean pipettes must be placed in the tray with the mouth ends facing the user

Cold water or detergent solution must be available in the work area to receive used or soiled pipettes

Usage of pipettes (4)

Zero mark

When filling the pipette, the meniscus is set at the:

Upper calibrations

The required amount should be delivered using the \__________________ which are more accurate than the lower calibrations

Smallest

Use the \_______ calibration pipette which will hold the volume desired