Untitled Flashcards Set

Culture media

Solid, liquid, or semi-solids used to support the growth of microorganisms.

Liquid

State of matter of culture media broth

Culture media

Solid, liquid, or semi-solids used to support the growth of microorganisms.

Liquid

State of matter of culture media broth

Solid, Liquid (broth), Semi-solid

Classifications of culture media according to consistency

1.5-3% of agar

Amount of solidifying agent in solid culture media

Agar

Most commonly used solidifying agent

Greater than or equal to 95℃

Melting point of agar

Less than 50℃

Temperature where agar resolidifies

Solid

Culture media used only to identify one type of specific microorganisms

Solid

Culture media used for observing colony appearance

Solid

Culture media used for pure culture isolations

Solid

Culture media used for storage cultures

Solid

Culture media used to observe specific biochemical reactions

Turbid

State of liquid broth when there is growth

Clear

State of liquid broth when there is no growth

Liquid/Broth

Culture media used to propagate large numbers of microorganisms

Liquid/Broth

Culture media for various biochemical tests

Less than 1.5% of agar

Amount of solidifying agent in semi-solid culture media

Semi-solid

Culture media used for fermentation studies

Semi-solid

Culture media used in determining bacterial motility

Semi-solid

Culture media promoting anaerobic growth

Semi-solid

Culture media using a wireloop or wirestick

Enrichment media

Culture media containing specific nutrients for specific pathogen growth

Nutrient specificity

Concept behind enrichment media

Selective media

Culture media favoring growth of desired bacteria while inhibiting undesired bacteria

Enriched medium

Culture media enriched by adding blood or albumin

Supportive/nonselective medium

Culture media supporting growth of most non-fastidious organisms

Differential medium

Culture media used to distinguish bacteria on the same agar plate

1. Weigh the powder 2. Dissolve 3. Dispense 4. Sterilize

Tubed media preparation steps

1. Weigh the powder 2. Dissolve 3. Sterilize 4. Dispense

Plated media preparation steps

Sterilization

Process of rendering media free of all forms of life

Autoclaving

Sterilization method using steam at 121C and 15lbs of pressure

121 C

Temperature of steam in autoclaving

15 lbs

Pressure used for autoclaving

UV radiation

Sterilization method using 260nm UV radiation

260nm

Wavelength of UV radiation in sterilization

Ethylene oxide

Sterilization method effective in killing microorganisms and spores

Crystal violet, gram's iodine, alcohol, safranin red

Stains used in gram staining

30 sec

Length of time crystal violet is used

1 min

Length of time gram's iodine is used

5-15 sec

Length of time alcohol is used in staining

1 min

Length of time safranin red is used

Colorless

Color of gram negative bacteria after alcohol application