Untitled Flashcards Set

Solid, liquid, or semi solids usually bought in reagent bottles in powdered form that are used to support the growth of microorganisms

-            Culture media

In what state of matter does culture media broth exist in

-            liquid

Classifications of culture media according to consistency

-            Solid, Liquid(broth), Semi-solid

How much solidifying agent is added to solid culture media

-            1.5-3% of agar

The most commonly used substance as a solidifying agent

-            Agar

melting point of agar

-            Greater than or equal to 95℃

Temperature where agar resolidifies

-            Less than 50 ℃

Culture media according to consistency that is used only to identify one type of specific microorganisms

-            Solid

Culture media according to consistency that is used for the surface growth of microorganisms to observe colony appearance

-            Solid

Culture media according to consistency that is used for pure culture isolations

-            Solid

Culture media according to consistency used for storage cultures

-            Solid

Culture media according to consistency used to observe specific biochemical reactions

-            Solid

What is the state of the liquid broth when there is growth

-            Turbid

What is the state of the liquid broth when there is no growth

-            Clear

Culture media according to consistency that can be used to propagate large numbers of microorganisms in fermentation tubes

-            Liquid/Broth

Culture media according to consistency that can be used for various biochemical tests

-            Liquid/Broth

How much solidifying agent is added to semi-solid culture media

-            less than 1.5% of agar

Culture media according to consistency that is used for fermentation studies

-            Semi-solid

Culture media according to consistency that is used in determining bacterial motility

-            Semi-solid

Culture media according to consistency used in promoting anaerobic growth

-            Semi-solid

Culture media according to consistency that uses a wireloop or wirestick

-            Semi-solid

Culture media according to function that contains specific nutrients for the enhancement of growth of a particular pathogen from a mixture of organisms

-            Enrichment media

Concept enrichment media uses to enhance the growth of a specific pathogen in a mixture of microorganisms through the nutrients it contains

-            Nutrient specificity

Culture media according to function that favors the growth of a particular desired bacteria by inhibiting the growth of undesired bacteria

-            Selective media

Culture media according to function that is enriched usually by adding blood or eggs(albumin)

-            Enriched medium

Culture media according to function that contains nutrients to support the growth of most non-fastidious organisms without giving any particular organism a growth advantage

-            Supportive/nonselective medium

Culture media according to function that is used to distinguish bacteria from each other on the same agar plate

-            Differential medium

Tubed media preparation steps(1-4)

1. Weigh the powder

2. Dissolve

3. Dispense

4. Sterilize

Plated media preparation steps(1-4)

1. Weigh the powder

2. Dissolve

3. Sterilize

4. Dispense

The process of rendering a medium or material free of all forms of life

-            Sterilization

Sterilization that exposes media to steam at 121C and 15lbs of pressure for 15mins or longer, depending on the nature of the item

-            Autoclaving

Temperature of the steam in autoclaving

-            121 C

Pressure used for autoclaving

-            15 lbs

Sterilization method that uses 260nm Ultra violet radiation, which is lethal to most microorgnaisms but does not penetrate glass, dirt films, water, and other substances effectively

-            UV radiation

wavelength of the UV radiation in sterilization

-            260nm

Sterilization method to kill both microorganisms and spores. It is particularly effective as a sterilizing agent because it rapidly penetrates packing materials, even plastic wraps

-            Ethylene oxide

The stains used in gram staining

-            Crystal violet, gram's iodine, alcohol, safranin red

length of time crystal violet is used

-            30 sec

length of time gram's iodine is used

-            1min

length of time alcohol is used in staining

-            5-15sec

length of time safranin red is used

-            1min

Color of gram negative bacteria once alcohol is used

-            Colorless