DNA replication

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4 Terms

1
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what was Meselson & Stahl’s experiment?

  • semi conservative replication.

    • bacteria grown in N15 - all DNA is heavy

    • transferred to N14 medium

    • continued growth in N14 medium

    • formed semi conservative strands

2
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what is the direction of replication?

  • bidirectional

  • 2 replication forks/origin

3
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where does replication begin in prokaryotes?

  • origin of replication is called OriC in prokaryotes

  • only one origin of replication in pro., multiple origins in eukaryotes

  • DNA replicated from one origin of replication = replicon

  • only one replicon in prokaryotes

  • Ter regions for termination of replication

4
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where does replication begin in eukaryotes?

  • multiple replicons in eukaryotes DNA replicated

  • origins 3 and 5 fire first, followed by 1

  • origins 2 and 4 are replicated by the elongation of replication forms from origins 3 and 5

  • origins 2 and 4 are passively replicated