micto bb

Autoclaving can achieve sterilization because it is lethal to

vriuses

spores

vegetable cells

Why are slides used for the capsule stain not subjected to normal heat fixing?

Heat fixing can destroy the capsule.

Where would a capsule be found on a bacterial cell?

on top of the cell wall

Why is flaming with the Bunsen burner a part of aseptic technique?

It destroys spores on equipment (loops, needles, bottles, tubes, etc).

Which species below would be most suitable for use in the spore stain?

Clostridium acetobutylicum

a patient is suspected of having tuberculosis, which stain would be more useful?

acid fast stain

Both differential stains described in this exercise detect differences in which cellular structure?

cell wall

What type of cultures are best as the sounce of cells for Gram staining?

Fresh cultures (16-18 hours old)

Which substance below is unique to acid-fast bacterial species?

mycolic acids

Which substance below is unique to gram-negative bacterial species?

LPS

During heat fixing, slides should be passed through the base of the flame close to the burner.

true

When accessing a culture tube in order to aseptically retrieve cells or inoculate the medium, how is the cap handled?

Held in the pinky finger of the hand holding the loop or needle

Why are loops and needles cooled prior to retrieving cells?

Hot loops and needles can kill cells.

When is it appropriate to flame a loop or needle?

before entering a culture after entering a culture

During heat fixing, slides should be passed through the base of the flame close to the burner.

true

When accessing a culture tube in order to aseptically retrieve cells or inoculate the medium, how is the cap handled?

Held in the pinky finger of the hand holding the loop or needle

Why are loops and needles cooled prior to retrieving cells?

Hot loops and needles can kill cells.

When is it appropriate to flame a loop or needle?

before entering a culture / after entering a culture

You observe cells under the microscope using a wet mount or hanging drop slide. The cells appear to be swimming in an animated, directional manner. This is most consistent with

true motility

You observe cells under the microscope using a wet mount or hanging drop slide. All of the cells in your field of view move in a flowing, sweeping motion toward one side of the slide. This is most consistent with

water current movement

You observe cells under the microscope using a wet mount or hanging drop slide. When the cells move, they do so randomly with a jerky, vi-bratory, seemingly aimless movement. This is most consistent with

brownian movement

Which of the following is NOT part of a bacterial flagellum?

peptidoglycan

Which type of cell movement is required in order for a cell to display chemotaxis?

true motility (via flagella)