Lesson_20_-_Basic_Components_in_HPLC_system
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10 Terms
HPLC
High-Performance Liquid Chromatography, a separation technique for analyzing compounds.
Mobile Phase
The solvent that carries the sample through the column in HPLC.
Degasser
A component used to remove excess dissolved gas from the mobile phase.
Pump
The heart of the HPLC system, responsible for driving the mobile phase and sample to the column.
Injector
A part of HPLC where the sample is introduced, typically using a loop system.
Column Oven
Maintains constant temperature to affect the separation of different compounds.
Pressure Unit
Measurements used in HPLC, including psi and bar, indicating the pressure generated by the pump.
Separation
The process in which sample components are separated based on their interaction with the mobile and stationary phases.
Stationary Phase
The surface (particles or film) over which the mobile phase and sample components flow for separation.
Safety Precautions
Measures taken to handle toxic solvents, prevent pulmonary irritation, and other high-pressure risks in an HPLC lab.
Flashcards (27)